{"id":52740,"date":"2024-11-11T08:07:06","date_gmt":"2024-11-11T00:07:06","guid":{"rendered":"https:\/\/www.chemhui.com\/?p=52740"},"modified":"2024-11-01T16:07:42","modified_gmt":"2024-11-01T08:07:42","slug":"nat-chem-bio-%ce%bcmap%e9%82%bb%e8%bf%91%e6%a0%87%e8%ae%b0%e6%8a%80%e6%9c%af%e6%8f%ad%e7%a4%ba%e6%b4%bb%e7%bb%86%e8%83%9e%e4%b8%ad%e5%ba%94%e6%bf%80%e9%a2%97%e7%b2%92%e7%9a%84%e5%88%86%e8%a7%a3","status":"publish","type":"post","link":"http:\/\/www.chemhui.com\/nat-chem-bio-%ce%bcmap%e9%82%bb%e8%bf%91%e6%a0%87%e8%ae%b0%e6%8a%80%e6%9c%af%e6%8f%ad%e7%a4%ba%e6%b4%bb%e7%bb%86%e8%83%9e%e4%b8%ad%e5%ba%94%e6%bf%80%e9%a2%97%e7%b2%92%e7%9a%84%e5%88%86%e8%a7%a3\/","title":{"rendered":"Nat. Chem. Bio. | \u03bcMap\u90bb\u8fd1\u6807\u8bb0\u6280\u672f\u63ed\u793a\u6d3b\u7ec6\u80de\u4e2d\u5e94\u6fc0\u9897\u7c92\u7684\u5206\u89e3\u673a\u5236"},"content":{"rendered":"<p>\u5206\u4eab\u4e00\u7bc7\u53d1\u8868\u5728Nature chemical biology\u4e0a\u7684\u6587\u7ae0\uff0c\u6587\u7ae0\u7684\u9898\u76ee\u662f\u201c\u00b5Map proximity labeling in living cells reveals stress granule disassembly mechanisms\u201d\uff0c\u4f5c\u8005\u662f\u6765\u81ea\u666e\u6797\u65af\u987f\u5927\u5b66\u6770\u51fa\u5316\u5b66\u6559\u6388David W. C. MacMillan\u3002\u5176\u7814\u7a76\u9886\u57df\u4e3b\u8981\u5305\u542b\u6709\u673a\u50ac\u5316\u3001\u7ea7\u8054\u50ac\u5316\u3001\u534f\u540c\u50ac\u5316\u3001\u5149\u8fd8\u539f\u50ac\u5316\u548c\u91d1\u5c5e\u5149\u8fd8\u539f\u50ac\u5316\u53ca\u5176\u5728\u5929\u7136\u4ea7\u7269\u548c\u836f\u7269\u7684\u5168\u5408\u6210\u4e2d\u7684\u5e94\u7528\u3002\u5229\u7528\u5149\u50ac\u5316\u7814\u7a76\u590d\u6742\u7684\u751f\u7269\u73af\u5883\u3002<\/p>\n<p>&nbsp;<\/p>\n<section><img decoding=\"async\" title=\"1\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20241017\/1729150976505444.png\" alt=\"1\" \/><\/section>\n<section><\/section>\n<section><strong>\u80cc\u666f\uff1a<\/strong><\/section>\n<section>\u7ec6\u80de\u8fc7\u7a0b\u901a\u8fc7\u591a\u79cd\u7ed3\u6784\u7684\u9694\u5ba4\u5316\u8fdb\u884c\u8c03\u63a7\uff0c\u5176\u4e2d\u5305\u62ec\u901a\u8fc7\u6db2-\u6db2\u76f8\u5206\u79bb\u5f62\u6210\u7684\u65e0\u819c\u7ec6\u80de\u5668\u3002\u8fd9\u6837\u7684<span 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class=\"\">\u4e9a\u7837\u9178\u94a0<\/span>\u5904\u7406\u5e94\u6fc0\uff08AS\uff1b500\u00b5M NaAsO2\uff09\u3002\u901a\u8fc7\u514d\u75ab\u8367\u5149\u6280\u672f\uff0c\u4f5c\u8005\u9a8c\u8bc1\u4e86\u5728\u5e94\u7528AS\u65f6\uff0c\u751f\u7269\u7d20\u5316\u4fe1\u53f7\u4e0eHaloTag\u2013G3BP1\u7684\u7ec6\u80de\u8d28\u9897\u7c92\u7ed3\u6784\u7684\u5171\u5b9a\u4f4d\uff0c\u8868\u660e\u8fd9\u4e9b\u76f8\u5206\u79bb\u7ed3\u6784\u7684\u9ad8\u5206\u8fa8\u7387\u90bb\u8fd1\u6807\u8bb0\uff08\u56fe1c\uff09\u3002<\/section>\n<section>\u63a5\u4e0b\u6765\uff0c\u4f5c\u8005\u5229\u7528label-free<span class=\"\">\u5b9a\u91cf\u86cb\u767d\u7ec4\u5b66<\/span>\u68c0\u6d4bSG\u86cb\u767d\u7ec4\u6210\u53d8\u5316\u3002\u4f5c\u8005\u8bc6\u522b\u51fa371\u4e2a\u5047\u5b9a\u7684G3BP1\u76f8\u4e92\u4f5c\u7528\u8005\u4f5c\u4e3a\u5e94\u6fc0\u8bf1\u5bfc\u9897\u7c92\u5019\u9009\u8005\uff08\u56fe1d\uff09\u3002\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u8005\u4e0e\u5148\u524d\u62a5\u9053\u7684 SG \u86cb\u767d\u5177\u6709\u5f88\u5927\u7684\u91cd\u53e0\u6027\uff0c\u800c\u6700\u9ad8\u5ea6\u5bcc\u96c6\u7684\u5019\u9009\u8005\uff08NUFIP2\uff0cUBAP2L\uff0cCAPRIN1\u7b49\uff09\u4e00\u8d2f\u88ab\u8ba4\u4e3a\u662f\u9897\u7c92\u7684\u6838\u5fc3\u7ed3\u6784\u7ec4\u5206\uff08\u56fe1d\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u6709\u6548\u9a8c\u8bc1\u4e86\u65b9\u6cd5\u7684\u51c6\u786e\u6027\uff0c\u5e76\u8bc1\u5b9e\u4e86\u4e4b\u524d\u5173\u4e8eAS\u8bf1\u5bfc\u9897\u7c92\u7ec4\u6210\u7684\u7814\u7a76\u3002\u540c\u65f6\uff0c\u4f5c\u8005\u4e5f\u9274\u5b9a\u5230\u4e4b\u524d\u5e76\u672a\u62a5\u9053\u7684\u86cb\u767d\u3002\u4f5c\u8005\u901a\u8fc7\u514d\u75ab\u8367\u5149\u6280\u672f\u786e\u8ba4\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u5728AS\u4e0b\u5b9a\u4f4d\u4e8eSGs\uff08\u56fe1e\uff09\u3002G3BP1\u53ca\u82e5\u5e72\u76f8\u5173SG\u86cb\u767d\u4e5f\u88ab\u8ba4\u4e3a\u5728\u6b63\u5e38\u6761\u4ef6\u4e0b\u9884\u5148\u76f8\u4e92\u4f5c\u7528\u3002\u4f5c\u8005\u5047\u8bbeHaloMap\u4e5f\u53ef\u4ee5\u5206\u6790\u8fd9\u4e9b\u5e94\u6fc0\u524d\u76f8\u4e92\u4f5c\u7528\u3002\u4f5c\u8005\u6bd4\u8f83\u4e86\u5728\u6ca1\u6709\u5e94\u6fc0\u4e0bHaloTag\u2013G3BP1\u7684\u76f8\u4e92\u4f5c\u7528\u7ec4\uff0c\u5e76\u8bc6\u522b\u51fa14\u4e2a\u9ad8\u5ea6\u5bcc\u96c6\u7684\u86cb\u767d\uff0c\u5305\u62ecUSP10\uff0cG3BP2\uff0cFXR2\u548cYTHDF3\u7b49\uff08\u56fe1f\uff09\u3002\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u88ab\u63a8\u6d4b\u4e0eG3BP1\u5f62\u6210\u201c\u9884\u7f6e\u201d\u590d\u5408\u7269\uff0c\u53ef\u80fd\u5728SGs\u7684\u5feb\u901f\u5f62\u6210\u4e2d\u8d77\u4f5c\u7528\u3002\u603b\u4e4b\uff0c\u8fd9\u4e9b\u53d1\u73b0\u8868\u660e\u7ec6\u80de\u5185HaloMap\u53ef\u4ee5\u6709\u6548\u4e14\u51c6\u786e\u5730\u6355\u83b7\u4e0d\u5e94\u6fc0\u6216\u5e94\u6fc0\u8bf1\u5bfc\u7684G3BP1\/SG\u76f8\u4e92\u4f5c\u7528\u7ec4\u3002<\/section>\n<section><img decoding=\"async\" title=\"2\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20241017\/1729150988620284.png\" alt=\"2\" \/><\/section>\n<section>\u56fe1 HaloMap\u65b9\u6cd5\u7684\u793a\u610f\u56fe\u3002\u00a0 \u00a0<\/section>\n<section>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/section>\n<section><strong>2.HaloMap\u65b9\u6cd5\u9274\u5b9a\u6cdb\u7d20\u4ecb\u5bfc\u7684SG\u89e3\u805a\u901a\u8def<\/strong><\/section>\n<section>\u5728\u5efa\u7acb\u4e86HaloMap\u6807\u8bb0\u5e73\u53f0\u540e\uff0c\u4f5c\u8005\u63a5\u4e0b\u6765\u8bd5\u56fe\u5206\u6790SG\u89e3\u805a\u8fc7\u7a0b\u4e2d\u53d1\u751f\u7684\u86cb\u767d\u7ec4\u5b66\u53d8\u5316\uff0c\u4ee5\u6355\u6349\u53ef\u80fd\u7684\u5173\u952e\u8c03\u8282\u56e0\u5b50\u3002SG\u5728\u65b0\u9c9c\u57f9\u517b\u57fa\u4e2d\u7ea62\u5c0f\u65f6\u540e\u5b8c\u5168\u89e3\u805a\uff08\u56fe2a\uff09\u3002\u57fa\u4e8e\u8fd9\u4e9b\u52a8\u6001\u5b66\uff0c\u4f5c\u8005\u5728\u53bb\u9664AS\u540e\u768430\u5206\u949f\uff08D30\uff09\u300160\u5206\u949f\uff08D60\uff09\u548c120\u5206\u949f\uff08D120\uff09\u8fdb\u884cHaloMap\u6807\u8bb0\uff0c\u4ee5\u4ee3\u8868\u89e3\u805a\u8fc7\u7a0b\u7684\u65e9\u671f\u3001\u4e2d\u671f\u548c\u665a\u671f\uff08\u56fe2b\uff09\u3002\u4ece\u6bcf\u4e2a\u65f6\u95f4\u70b9\u7684HaloTag-G3BP1\u4e0eHaloTag-NES\u6807\u8bb0\u6bd4\u8f83\u4e2d\uff0c\u9274\u5b9a\u5230698\u4e2aSG\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u6c34\u5e73\u53d8\u5316\u3002\u968f\u7740\u9897\u7c92\u7684\u89e3\u805a\uff0c\u5927\u7ea670%\u7684\u9897\u7c92\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u7684\u5bcc\u96c6\u968f\u7740\u6062\u590d\u800c\u964d\u4f4e\uff08\u56fe2c\uff09\u3002\u7136\u800c\uff0c\u4f5c\u8005\u5bf9\u4e00\u7ec4\u5728\u6062\u590d\u671f\u95f4\u5448\u73b0\u51fa\u589e\u52a0\u5173\u8054\u7684\u86cb\u767d\u8d28\uff08n=203\uff09\u7684\u51fa\u73b0\u611f\u5230\u5174\u8da3\uff0c\u56e0\u4e3a\u8fd9\u4e9b\u53ef\u80fd\u662f\u9897\u7c92\u89e3\u805a\u7684\u8c03\u63a7\u86cb\u767d\u3002GO\u5206\u6790\u663e\u793a\u51fa\u660e\u663e\u5bcc\u96c6\u7684\u86cb\u767d\u8d28\uff0c\u4e3b\u8981\u662f\u6b63\u5411\u8c03\u8282\u6216\u542f\u52a8\u7ffb\u8bd1\u7684\u86cb\u767d\u8d28\uff0c\u5373\u968f\u7740\u7ec6\u80de\u4ece\u5e94\u6fc0\u4e2d\u6062\u590d\uff0cmRNA\u4ece\u9897\u7c92\u7684\u91ca\u653e\u548c\u7ffb\u8bd1\u3002\u6b64\u5916\uff0c\u591a\u4e2a\u4e0e\u6cdb\u7d20\u5316\u548c\u86cb\u767d\u8d28\u8d28\u91cf\u63a7\u5236\u9014\u5f84\u76f8\u5173\u7684\u86cb\u767d\u8d28\u4e5f\u663e\u793a\u51fa\u5bcc\u96c6\uff08\u56fe2c\uff09\u3002\u63a5\u4e0b\u6765\uff0c\u901a\u8fc7\u514d\u75ab\u8367\u5149\uff0c\u4f5c\u8005\u89c2\u5bdf\u5230\u5728\u5e94\u6fc0\u5904\u7406\u540e\uff0c\u6cdb\u7d20\u4fe1\u53f7\u4e0eG3BP1\/\u9897\u7c92\u7684\u5f3a\u91cd\u53e0\uff0c\u8fd9\u8868\u660e\u8fd9\u4e9b\u51dd\u805a\u7269\u542b\u6709\u591a\u6cdb\u7d20\u5316\u7684\u86cb\u767d\u3002\u6b64\u5916\uff0c\u4f5c\u8005\u786e\u8ba4\u5728\u5e94\u6fc0\u548c\u65e9\u671f\u6062\u590d\u65f6\uff08\u5e94\u6fc0\u540e30\u5206\u949f\uff09\u6574\u4f53\u591a\u6cdb\u7d20\u6c34\u5e73\u7684\u4e0a\u8c03\uff08\u56fe2e\uff09\u3002\u4e3a\u4e86\u8fdb\u4e00\u6b65\u4e86\u89e3\u6cdb\u7d20\u5316\u5bf9\u9897\u7c92\u52a8\u529b\u5b66\u7684\u5f71\u54cd\uff0c\u4f5c\u8005\u63a5\u4e0b\u6765\u6d4b\u8bd5\u4e86\u5728\u5b58\u5728TAK-243\uff08MLN7243\uff09\uff0c\u4e00\u79cd\u5f3a\u6548\u7684\u6cdb\u7d20\u6fc0\u6d3b\u9176\uff08UAE\uff09\u6291\u5236\u5242\u7684\u60c5\u51b5\u4e0b\uff0c\u51dd\u805a\u7269\u7684\u5f62\u6210\u548c\u89e3\u805a\uff08\u56fe2f\uff09\u3002\u4f5c\u8005\u5728\u4e0d\u540c\u65f6\u95f4\u6bb5\u6291\u5236\u6cdb\u7d20\u5316\uff081\uff09\u5728\u9897\u7c92\u5f62\u6210\u548c\u89e3\u805a\u7684\u6574\u4e2a\u5468\u671f\uff08TAK-243-1\uff09\uff0c\uff082\uff09\u5728\u9897\u7c92\u5f62\u6210\u671f\u95f4\uff08TAK-243-2\uff09\uff0c\u4ee5\u53ca\uff083\uff09\u4ec5\u5728\u89e3\u805a\u671f\u95f4\uff08TAK-243-3\uff1b\u56fe2g\uff09\u3002\u8fd9\u4e9b\u6bd4\u8f83\u7ed3\u679c\u663e\u793a\uff0c\u5728\u4efb\u4f55\u4e00\u4e2a\u65f6\u671f\uff08\u7ec41\uff0c2\u62163\uff09\u6291\u5236\u6cdb\u7d20\u5316\u5bf9\u9897\u7c92\u5f62\u6210\u7684\u5f71\u54cd\u8f83\u5c0f\u3002\u7136\u800c\uff0c\u5f53\u5728\u9897\u7c92\u5f62\u6210\u671f\u95f4\u6291\u5236\u6cdb\u7d20\u5316\u65f6\uff08TAK-243-1\u548cTAK-243-2\uff09\u89c2\u5bdf\u5230\u4e86\u663e\u8457\u5ef6\u8fdf\u7684\u9897\u7c92\u89e3\u805a\uff0c\u800c\u5728\u4ec5\u5728\u89e3\u805a\u671f\u95f4\u65bd\u7528TAK-243\u65f6\uff08TAK-243-3\uff09\u5219\u89c2\u5bdf\u5230\u4e86\u8f7b\u5fae\u7684\u5ef6\u8fdf\uff08\u56fe2h\uff09\u3002\u603b\u7684\u6765\u8bf4\uff0c\u8fd9\u4e9b\u7ed3\u679c\u5f3a\u70c8\u8868\u660e\u6cdb\u7d20\u5316\u5bf9\u9897\u7c92\u89e3\u805a\u81f3\u5173\u91cd\u8981\u3002\u00a0 \u00a0<\/section>\n<section><img decoding=\"async\" title=\"3\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20241017\/1729151000351365.png\" alt=\"3\" \/><\/section>\n<section>\u56fe2 HaloMap\u5206\u6790\u6cdb\u7d20\u5316\u5bf9\u4e8eSG\u89e3\u805a\u662f\u5173\u952e\u7684\u4fee\u9970\u3002<\/section>\n<section><\/section>\n<section><strong>3.\u76f8\u4e92\u4f5c\u7528\u7ec4\u56fe\u8c31 (+\/\u2212)TAK-243 \u786e\u5b9a\u5019\u9009\u8c03\u8282\u56e0\u5b50<\/strong><\/section>\n<section>\u4f5c\u8005\u56e0\u6b64\u5728HaloTag\u2013G3BP1\u7a33\u5b9a\u7ec6\u80de\u7cfb\u4e0a\u5bf9\u6bd4\u4e86\u6709\u65e0TAK-243\u5904\u7406\u4e0b\u7684\u7ec6\u80de\u5728\u5e94\u6fc0\u524d\u53ca\u6574\u4e2a\u89e3\u805a\u8fc7\u7a0b\u4e2d\u7684\u60c5\u51b5\uff08\u56fe3a\uff09\u3002\u9274\u5b9a\u5230\u7531TAK-243\u6291\u5236\u591a\u805a\u6cdb\u7d20\u5316\u6240\u89e6\u53d1\u7684\u9897\u7c92\u86cb\u767d\u7ec4\u5dee\u5f02\u3002\u4f5c\u8005\u805a\u7126\u4e8e\u4e0e\u6cdb\u7d20\u901a\u8def\u76f8\u5173\u7684\u5dee\u5f02\u5bcc\u96c6\u86cb\u767d\uff0c\u9996\u5148\u641c\u7d22\u4e86E3\u8fde\u63a5\u9176\uff0c\u8bc6\u522b\u51fa\u4e86\u5728\uff08+\uff09TAK-243\u7ec6\u80de\u4e2d\u4e8e\u5e94\u6fc0\u540e\u5bcc\u96c6\u7684\u51e0\u79cd\u5019\u9009\u86cb\u767d\uff08\u56fe3b\u3001c\uff09\u3002\u5176\u4e2d\u5305\u62ecE3\u8fde\u63a5\u9176\uff0c\u8bc6\u522b\u6cdb\u7d20\u7ffb\u8bd1\u540e\u4fee\u9970\uff08PTMs\uff09\u7684\u86cb\u767d\u4ee5\u53ca\u4f5c\u4e3a\u9897\u7c92\u89e3\u805a\u7684\u86cb\u767d\u3002\u4f5c\u8005\u8fd8\u8bc6\u522b\u51fa\u51e0\u79cd\u4e0e\u81ea\u566c\u76f8\u5173\u7684\u6cdb\u7d20\u53d7\u4f53\uff0c\u5305\u62ecSQSTM1\u3001HDAC6\u548cTOLLIP\uff0c\u6240\u6709\u8fd9\u4e9b\u86cb\u767d\u90fd\u5df2\u88ab\u8bc1\u660e\u80fd\u591f\u8bc6\u522b\u805a\u6cdb\u7d20\u5316\u5e95\u7269\u4ee5\u8fdb\u884c\u81ea\u566c\u9776\u5411\u548c\u964d\u89e3\uff08\u56fe3d\u3001e\uff09\u3002<\/section>\n<section><img decoding=\"async\" title=\"4\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20241017\/1729151012954617.png\" alt=\"4\" \/>\u56fe3 \u9897\u7c92\u86cb\u767d\u7ec4\u5728\u5e94\u6fc0\u524d\u5230\u665a\u671f\u89e3\u4f53\u72b6\u6001\u4e2d\u7684\u53d8\u5316\uff0c\u5305\u62ec\u6709\uff08+\uff09\u548c\u6ca1\u6709\uff08-\uff09TAK-243\u7684\u60c5\u51b5\u3002<\/section>\n<section><\/section>\n<section><strong>4.HECT E3\u8fde\u63a5\u9176\u4fc3\u8fdb\u9897\u7c92\u89e3\u4f53<\/strong><\/section>\n<section>\u4f5c\u8005\u5728HaloMap\u5b9e\u9a8c\u4e2d\u53d1\u73b0HECT E3\u8fde\u63a5\u9176\u7684\u5bcc\u96c6\u3002\u4e3a\u4e86\u7814\u7a76HECT E3\u8fde\u63a5\u9176\u662f\u5426\u5728SG\u7684\u89e3\u4f53\u4e2d\u8d77\u4f5c\u7528\uff0c\u4f5c\u8005\u5728\u523a\u6fc0\u548c\u6062\u590d\u671f\u95f4\u7528HECT\u8fde\u63a5\u9176\u7279\u5f02\u6027\u6291\u5236\u5242heclin\u5904\u7406\u7ec6\u80de\u3002\u5728120\u5206\u949f\u7684\u6062\u590d\u671f\uff08D120\uff09\uff0checlin\u5904\u7406\u7684\u6837\u672c\u4e2d\u4ecd\u7136\u6b8b\u7559\u5927\u91cf\u7684\u9897\u7c92\uff08\u56fe4a\uff0cb\uff09\u3002\u4f5c\u8005\u63a5\u4e0b\u6765\u63a2\u7d22\u4e86\u7279\u5b9a\u7684HECT E3\u8fde\u63a5\u9176\u6216\u5176\u76f8\u5173\u7ed3\u5408\u86cb\u767d\u662f\u5426\u8c03\u8282SG\uff08\u5e94\u6fc0\u9897\u7c92\uff09\u7684\u89e3\u4f53\u3002\u4e3a\u6b64\uff0c\u4f5c\u8005\u5bf9\u5728HaloMap\u4e2d\u8bc6\u522b\u7684\u6bcf\u4e2a\u5019\u9009\u86cb\u767d\u8fdb\u884c\u4e86siRNA\u6572\u4f4e\u5b9e\u9a8c\uff0c\u5305\u62ec\u6240\u6709\u56db\u79cd\u8fde\u63a5\u9176\uff08WWP2\u3001ITCH\u3001NEDD4\u548cNEDD4L\uff09\u4ee5\u53ca\u4e24\u4e2a\u7ed3\u5408\u86cb\u767d\uff08NDFIP1\u548cNDFIP2\uff09\u3002\u5355\u72ec\u6572\u4f4eITCH\u3001NEDD4L\u548cNDFIP1\u5bfc\u81f4SG\u89e3\u4f53\u7684\u660e\u663e\u5ef6\u8fdf\u3002\u9274\u4e8e\u8fd9\u4e9b\u8fde\u63a5\u9176\u4e4b\u524d\u672a\u4e0e\u9897\u7c92\u76f8\u5173\u8054\uff0c\u4f5c\u8005\u901a\u8fc7\u9488\u5bf9\u9897\u7c92\u6807\u8bb0\u7269G3BP1\u7684\u514d\u75ab\u8367\u5149\u786e\u8ba4\u4e86\u5b83\u4eec\u7684\u4e9a\u7ec6\u80de\u5b9a\u4f4d\uff0c\u89c2\u5bdf\u5230\u5b83\u4eec\u5728NaAsO2\u5904\u7406\u540e\u88ab\u62db\u52df\u5230SG\u4e2d\uff08\u56fe4e\uff09\u3002\u8fc7\u8868\u8fbe\u6216siRNA\u6572\u4f4eITCH\u6216NEDD4L\u5747\u672a\u5f71\u54cd\u521d\u59cb\u7684\u9897\u7c92\u5f62\u6210\uff0c\u8fd9\u4e0e\u4f5c\u8005\u5bf9TAK-243\u548checlin\u6291\u5236\u591a\u805a\u6cdb\u7d20\u5316\u7684\u7ed3\u679c\u4e00\u81f4\uff08\u56fe4e\uff09\u3002\u7efc\u5408\u8fd9\u4e9b\u6570\u636e\uff0c\u8868\u660e\u7279\u5b9a\u7684HECT 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10.1038\/s41589-024-01721-2<\/section>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5206\u4eab\u4e00\u7bc7\u53d1\u8868\u5728Nature chemical biology\u4e0a\u7684\u6587\u7ae0\uff0c\u6587\u7ae0\u7684\u9898\u76ee\u662f\u201c\u00b5Map proximit 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