{"id":52078,"date":"2024-08-19T08:38:51","date_gmt":"2024-08-19T00:38:51","guid":{"rendered":"https:\/\/www.chemhui.com\/?p=52078"},"modified":"2024-08-10T21:41:30","modified_gmt":"2024-08-10T13:41:30","slug":"angew-chem-%ef%bc%9a%e7%bb%86%e8%83%9e%e8%89%b2%e7%b4%a0p450%e9%85%b6%e5%82%ac%e5%8c%96%e7%b4%ab%e6%9d%89%e9%86%87%e5%9b%9b%e5%85%83%e6%b0%a7%e7%8e%af%e7%9a%84%e5%bd%a2%e6%88%90","status":"publish","type":"post","link":"http:\/\/www.chemhui.com\/angew-chem-%ef%bc%9a%e7%bb%86%e8%83%9e%e8%89%b2%e7%b4%a0p450%e9%85%b6%e5%82%ac%e5%8c%96%e7%b4%ab%e6%9d%89%e9%86%87%e5%9b%9b%e5%85%83%e6%b0%a7%e7%8e%af%e7%9a%84%e5%bd%a2%e6%88%90\/","title":{"rendered":"Angew. Chem. \uff1a\u7ec6\u80de\u8272\u7d20P450\u9176\u50ac\u5316\u7d2b\u6749\u9187\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210"},"content":{"rendered":"<section>\n<section>\n<section>\n<section>\n<section><span class=\"wx_search_keyword_wrap\">\u7d2b\u6749\u9187<\/span>\u4e3a\u4e34\u5e8a\u4e0a\u6cbb\u7597\u591a\u79cd\u6076\u6027\u80bf\u7624\u6700\u6709\u6548\u7684\u5316\u7597\u836f\u7269\u4e4b\u4e00\uff0c\u9700\u6c42\u91cf\u5de8\u5927\u3002\u7531\u4e8e\u5176\u5929\u7136\u542b\u91cf\u4f4e\u4e14\u5316\u5b66\u5408\u6210\u56f0\u96be\uff0c\u836f\u6e90\u7d27\u5f20\uff0c\u4ef7\u683c\u6602\u8d35\u3002\u5f53\u524d\uff0c\u7d2b\u6749\u9187\u7684\u751f\u7269\u5408\u6210\u88ab\u8ba4\u4e3a\u662f\u4e00\u6761\u6709\u6548\u89e3\u51b3\u5176\u8d44\u6e90\u7d27\u7f3a\u7684\u53ef\u884c\u7b56\u7565\u3002\u5c3d\u7ba1\u7d2b\u6749\u9187\u7684\u751f\u7269\u5408\u6210\u5df2\u7ecf\u53d6\u5f97\u4e86\u5de8\u5927\u8fdb\u5c55\uff0c\u4f46\u5176\u5b8c\u6574\u751f\u7269\u5408\u6210\u9014\u5f84\u81f3\u4eca\u5c1a\u672a\u5b8c\u6210\u89e3\u6790\uff0c\u5c24\u5176\u662f\u4ecb\u5bfc\u5f62\u6210\u7d2b\u6749\u9187\u5206\u5b50\u4e2d\u7279\u5f81\u6027\u836f\u6548\u57fa\u56e2\u2014\u56db\u5143\u6c27\u73af\u7ed3\u6784\u5355\u5143 (oxetane ring) \u76f8\u5173\u9176\u53ca\u5176\u9176\u5b66\u673a\u5236\u81f3\u4eca\u5c1a\u4e0d\u5341\u5206\u6e05\u6670\uff0c\u4ea6\u662f\u5b66\u672f\u754c\u8ba8\u8bba\u7684\u70ed\u70b9\u3002<\/p>\n<p>\u8fd1\u65e5\uff0c\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u836f\u7269\u7814\u7a76\u6240\u6234\u5747\u8d35\u6559\u6388\u56e2\u961f\u4ece\u7a33\u4ea7\u7d2b\u6749\u9187\u7684<span class=\"wx_search_keyword_wrap\">\u66fc\u5730\u4e9a\u7ea2\u8c46\u6749<\/span>\u6108\u4f24\u7ec4\u7ec7\u4e2d\u53d1\u73b0\u5e76\u529f\u80fd\u9274\u5b9a\u4e86\u8d1f\u8d23\u50ac\u5316\u5f62\u6210\u7d2b\u6749\u9187\u56db\u5143\u6c27\u73af\u7684\u56db\u5143\u6c27\u73af\u5408\u9176oxetane synthase (TmCYP1)\uff0c\u901a\u8fc7\u5173\u952e\u5e95\u7269\u9972\u5582\u3001\u540c\u4f4d\u7d20\u6807\u8bb0 (\u6c18\u6807\u8bb0\u548c18O\u6807\u8bb0)\u5b9e\u9a8c\uff0c\u7ed3\u5408<span class=\"wx_search_keyword_wrap\">\u5316\u5b66\u8ba1\u7b97<\/span>\u7b49\u624b\u6bb5\u7cfb\u7edf\u9610\u660e\u4e86\u8be5P450\u9176\u4ecb\u5bfc\u7d2b\u6749\u9187\u56db\u5143\u6c27\u73af\u5f62\u6210\u7684\u9176\u5b66\u673a\u5236\uff0c\u4e3a\u7406\u89e3\u7d2b\u6749\u9187\u7684\u751f\u7269\u5408\u6210\u63d0\u4f9b\u4e86\u79d1\u5b66\u4f9d\u636e\uff0c\u4ea6\u4e3a\u5b9e\u73b0\u7d2b\u6749\u9187\u751f\u7269\u5408\u6210\u9014\u5f84\u7684\u5b8c\u5168\u89e3\u6790\u548c\u5f02\u6e90\u91cd\u6784\u5960\u5b9a\u4e86\u57fa\u7840\u3002<\/p>\n<p>\u8be5\u7814\u7a76\u901a\u8fc7\u5316\u5b66\u5408\u6210\u4e24\u4e2a\u5173\u952e\u5e95\u72691\u548c2\u5bf9\u56db\u5143\u6c27\u73af\u5408\u9176TmCYP1\u8fdb\u884c\u4e86\u529f\u80fd\u9274\u5b9a,\u53d1\u73b0TmCYP1\u80fd\u50ac\u5316\u5e95\u72691\u751f\u6210\u4e00\u4e2a\u4e3b\u8981\u56db\u5143\u6c27\u73af\u884d\u751f\u72691a\u548c\u4e00\u4e2a\u6b21\u89814(20)-\u73af\u6c27\u5316\u4ea7\u72691b;\u800cTmCYP1\u50ac\u5316\u5e95\u72692\u4ec5\u80fd\u4ea7\u751f4(20)-\u73af\u6c27\u5316\u4ea7\u72692b\u3002\u7531\u6b64\u5f97\u51fa\uff1a\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210\u9700\u8981C5\u4f4d\u4e59\u9170\u57fa\u7684\u53c2\u4e0e;C5\u4f4d\u4e59\u9170\u57fa\u5b58\u5728\u6216\u4e0d\u5b58\u5728\uff0c\u8be5\u9176\u5747\u53ef\u50ac\u53164(20)-\u53cc\u952e\u7684<span class=\"wx_search_keyword_wrap\">\u73af\u6c27\u5316\u53cd\u5e94<\/span>\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"1\" src=\"https:\/\/10110.seohost.cn\/storage\/10110\/article\/20240810\/1723297192903691.webp\" alt=\"1\" \/><\/section>\n<section>\u56fe1. \u56db\u5143\u6c27\u73af\u5408\u9176TmCYP1\u529f\u80fd\u9274\u5b9a<\/p>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\u4e3a\u8fdb\u4e00\u6b65\u7814\u7a76\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210\u673a\u5236\uff0c\u8bbe\u8ba1\u4e86\u4e24\u7ec4\u540c\u4f4d\u7d20\u6807\u8bb0\u5b9e\u9a8c\uff1aC5\u4f4d\u4e59\u9170\u57fa\u7684\u7532\u57fa\u6c18\u6807\u8bb0\u5b9e\u9a8c\u548cC5\u4f4d\u4e59\u9170\u6c27\u57fa\u7684\u7fb0\u57fa\u6c27\u539f\u5b50<sup>18<\/sup>O\u6807\u8bb0\u5b9e\u9a8c\u3002\u901a\u8fc7\u672c\u6c0f\u70df\u8349\u5e95\u7269\u9972\u5582\u53d1\u73b0\u5e95\u7269\u4e2dC5\u4f4d\u6c18\u6807\u8bb0\u7684\u7532\u57fa\u8fc1\u79fb\u5230\u4e86\u4ea7\u7269\u7684C4\u4f4d\uff0c\u800c\u5e95\u7269\u4e2d\u7684C5\u4f4d<sup>18<\/sup>O\u6807\u8bb0\u7684\u7fb0\u57fa\u6c27\u539f\u5b50\u51fa\u73b0\u5728\u4ea7\u7269\u4e2d\u4e0eC4\u4f4d\u76f4\u63a5\u76f8\u8fde\u7684\u6c27\u539f\u5b50\u3002\u4e0a\u8ff0\u540c\u4f4d\u7d20\u6807\u8bb0\u5b9e\u9a8c\u76f4\u63a5\u8bc1\u660e\u4e86\u5728\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210\u8fc7\u7a0b\u4e2d\uff0cC5\u4f4d\u7684\u4e59\u9170\u6c27\u57fa\u8fc1\u79fb\u5230C4\u4f4d\uff0c\u5728\u6b64\u8fc7\u7a0b\u4e2dC5\u4e59\u9170\u6c27\u57fa\u7684\u4e24\u4e2a\u6c27\u539f\u5b50\u53d1\u751f\u4e86\u4e92\u6362\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"2\" src=\"https:\/\/10110.seohost.cn\/storage\/10110\/article\/20240810\/1723297204882734.webp\" alt=\"2\" \/><\/section>\n<section>\u56fe2. \u540c\u4f4d\u7d20\u6807\u8bb0\u5b9e\u9a8c<\/p>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\u57fa\u4e8e\u6b64\uff0c\u4f5c\u8005\u63d0\u51fa\u4e86\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210\u673a\u5236\uff1a\u5728\u56db\u5143\u6c27\u73af\u5408\u9176\u4f5c\u7528\u4e0b\uff0c4(20)-\u53cc\u952e\u548cC5-OAc\u4e4b\u95f4\u53d1\u751f\u6c27\u5316-\u91cd\u6392\uff0c\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210\u53ef\u80fd\u662f\u4e00\u6b65<span class=\"wx_search_keyword_wrap\">\u534f\u540c\u53cd\u5e94<\/span>\u3002\u4f5c\u8005\u8fdb\u4e00\u6b65\u901a\u8fc7DFT\u8ba1\u7b97\u9a8c\u8bc1\u4e86\u8be5\u673a\u5236\u7684\u5408\u7406\u6027\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"3\" src=\"https:\/\/10110.seohost.cn\/storage\/10110\/article\/20240810\/1723297215954196.webp\" alt=\"3\" \/><\/section>\n<section>\u56fe3. \u56db\u5143\u6c27\u73af\u5f62\u6210\u673a\u5236<\/p>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\u968f\u540e\uff0c\u4f5c\u8005\u8bbe\u8ba1\u5408\u6210\u4e86\u5176\u4ed66\u4e2a\u4e0d\u540c\u6c27\u5316\u6c34\u5e73\u7684\u5e95\u7269\u7528\u6765\u63a2\u8ba8\u56db\u5143\u6c27\u73af\u5408\u9176TmCYP1\u5e95\u7269\u8c31\uff0c\u53d1\u73b0\u8be5\u9176\u4ec5\u80fd\u50ac\u5316\u9ad8\u6c27\u5316\u6c34\u5e73\u7684\u5e95\u7269(\u81f3\u5c115\u4e2a\u6c27\u53d6\u4ee3\u7684\u7d2b\u6749\u4e8c\u70ef)\u3002\u8be5\u7ed3\u679c\u4ea6\u63d0\u793a\u7d2b\u6749\u9187\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210\u4f4d\u4e8e\u7d2b\u6749\u9187\u751f\u7269\u5408\u6210\u7684\u540e\u671f\u9636\u6bb5\uff0c\u4e0e\u81ea\u7136\u754c\u4e2d\u5b58\u5728\u7684\u7d2b\u6749\u70f7\u7c7b\u5316\u5408\u7269\u7ed3\u6784\u7279\u70b9\u4e00\u81f4\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"0\" src=\"https:\/\/10110.seohost.cn\/storage\/10110\/article\/20240810\/1723297229568264.webp\" alt=\"0\" \/>\u200b<\/section>\n<section>\u56fe4. \u56db\u5143\u6c27\u73af\u5408\u9176TmCYP1\u5e95\u7269\u8c31\u8003\u5bdf<\/p>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\u8be5\u7814\u7a76\u53d1\u73b0\u5e76\u529f\u80fd\u9274\u5b9a\u4e86\u8d1f\u8d23\u7d2b\u6749\u9187\u56db\u5143\u6c27\u73af\u5f62\u6210\u7684\u56db\u5143\u6c27\u73af\u5408\u9176\uff0c\u901a\u8fc7\u591a\u79cd\u624b\u6bb5\u9610\u660e\u4e86\u7d2b\u6749\u9187\u56db\u5143\u6c27\u73af\u7684\u5f62\u6210\u673a\u5236\uff0c\u4e3a\u7d2b\u6749\u9187\u7684\u5b8c\u6574\u751f\u7269\u5408\u6210\u9014\u5f84\u89e3\u6790\u548c\u5f02\u6e90\u91cd\u6784\u5960\u5b9a\u4e86\u57fa\u7840\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section>\n<section>\n<section>\n<section><strong>\u8bba<\/strong><strong>\u6587\u4fe1\u606f<\/strong><\/p>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>A Cytochrome P450 Enzyme Catalyses Oxetane Ring Formation in Paclitaxel Biosynthesis<\/p>\n<p>Dr. Changkang Li,\u00a0Xinxin Yin,\u00a0Dr. Shuai Wang,\u00a0Songyang Sui,\u00a0Dr. Jimei Liu,\u00a0Xincheng Sun,\u00a0Jinming Di,\u00a0Dr. Ridao Chen,\u00a0Dr. Dawei Chen,\u00a0Yaotian Han,\u00a0Dr. Kebo Xie,\u00a0Prof. Jungui Dai<\/p>\n<p>\u6587\u7ae0\u7684\u7b2c\u4e00\u4f5c\u8005\u4e3a\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u836f\u7269\u7814\u7a76\u6240\u535a\u58eb\u540e\u674e\u5e38\u5eb7\u535a\u58eb (\u73b0\u5df2\u5165\u9009\u201c\u534f\u548c\u9752\u5e74\u5b66\u8005\u652f\u6301\u8ba1\u5212\u201d) \u548c\u535a\u58eb\u751f\u5c39\u6b23\u6b23\uff0c\u901a\u8baf\u4f5c\u8005\u4e3a\u89e3\u53ef\u6ce2\u3001\u6234\u5747\u8d35\u7814\u7a76\u5458\u3002\u8be5\u9879\u76ee\u5f97\u5230\u4e86\u56fd\u5bb6\u91cd\u70b9\u7814\u53d1\u8ba1\u5212(2020YFA0908000)\u4ee5\u53ca\u533b\u79d1\u9662\u533b\u5b66\u4e0e\u5065\u5eb7\u79d1\u6280\u521b\u65b0\u5de5\u7a0b(No. 2021-I2M-1-029)\u7684\u652f\u6301\u3002<\/p>\n<p><em>Angewandte Chemie International Edition<\/em><\/p>\n<p>DOI:\u00a010.1002\/anie.202407070<\/p>\n<\/section>\n<\/section>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u7d2b\u6749\u9187\u4e3a\u4e34\u5e8a\u4e0a\u6cbb\u7597\u591a\u79cd\u6076\u6027\u80bf\u7624\u6700\u6709\u6548\u7684\u5316\u7597\u836f\u7269\u4e4b\u4e00\uff0c\u9700\u6c42\u91cf\u5de8\u5927\u3002\u7531\u4e8e\u5176\u5929\u7136\u542b\u91cf\u4f4e\u4e14\u5316\u5b66\u5408\u6210\u56f0\u96be\uff0c\u836f\u6e90\u7d27\u5f20\uff0c\u4ef7\u683c [&hellip;]<\/p>\n","protected":false},"author":326,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-52078","post","type-post","status-publish","format-standard","hentry","category-tuijianyuedu"],"_links":{"self":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/52078","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/users\/326"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/comments?post=52078"}],"version-history":[{"count":1,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/52078\/revisions"}],"predecessor-version":[{"id":52079,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/52078\/revisions\/52079"}],"wp:attachment":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/media?parent=52078"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/categories?post=52078"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/tags?post=52078"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}