{"id":51762,"date":"2024-07-03T08:43:06","date_gmt":"2024-07-03T00:43:06","guid":{"rendered":"https:\/\/www.chemhui.com\/?p=51762"},"modified":"2024-06-24T10:46:11","modified_gmt":"2024-06-24T02:46:11","slug":"nat-commun-%e4%b8%80%e7%a7%8d%e7%94%a8%e4%ba%8e%e6%8e%a2%e6%b5%8b%e7%9b%b4%e6%8e%a5%e8%9b%8b%e7%99%bd%e7%9b%b8%e4%ba%92%e4%bd%9c%e7%94%a8%e7%9a%84%e5%8f%af%e5%af%8c%e9%9b%86%e3%80%81%e5%8f%af","status":"publish","type":"post","link":"http:\/\/www.chemhui.com\/nat-commun-%e4%b8%80%e7%a7%8d%e7%94%a8%e4%ba%8e%e6%8e%a2%e6%b5%8b%e7%9b%b4%e6%8e%a5%e8%9b%8b%e7%99%bd%e7%9b%b8%e4%ba%92%e4%bd%9c%e7%94%a8%e7%9a%84%e5%8f%af%e5%af%8c%e9%9b%86%e3%80%81%e5%8f%af\/","title":{"rendered":"Nat. Commun.|\u4e00\u79cd\u7528\u4e8e\u63a2\u6d4b\u76f4\u63a5\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684\u53ef\u5bcc\u96c6\u3001\u53ef\u88c2\u89e3\u5e76\u4e14\u5177\u6709\u6f5c\u5728\u751f\u7269\u53cd\u5e94\u6027\u7684\u975e\u5929\u7136\u6c28\u57fa\u9178"},"content":{"rendered":"<section>\n<section>\u63a8\u8350\u4e00\u7bc7\u53d1\u8868\u5728Nature Communication\u4e0a\u7684\u6587\u7ae0\uff0c\u6587\u7ae0\u6807\u9898\u4e3a\u201cCharacterize direct protein interactions with enrichable, cleavable and latent bioreactive unnatural amino acids\u201d\uff0c\u7b2c\u4e00\u901a\u8baf\u4f5c\u8005\u4e3a\u6d59\u6c5f\u5927\u5b66\u751f\u547d\u79d1\u5b66\u7814\u7a76\u9662\u7684\u6768\u5175\u7814\u7a76\u5458\u3002\u6768\u5175\u7814\u7a76\u5458\u81f4\u529b\u4e8e\u4ee5\u751f\u7269\u8d28\u8c31\u3001\u5316\u5b66\u751f\u7269\u5b66\u4ee5\u53ca\u8ba1\u7b97\u86cb\u767d\u8d28\u7ec4\u5b66\u4e3a\u57fa\u7840\uff0c\u5f00\u53d1\u65b0\u7684<span class=\"wx_search_keyword_wrap\">\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790\u6280\u672f<\/span>\uff0c\u7528\u4e8e\u7814\u7a76\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u4ee5\u53ca\u86cb\u767d\u8d28\u7ffb\u8bd1\u540e\u4fee\u9970\u3002<\/p>\n<p>\u7814\u7a76\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\uff08PPI\uff09\u5bf9\u4e8e\u63ed\u793a\u9776\u86cb\u767d\u7684\u529f\u80fd\u4ee5\u53ca\u7cfb\u7edf\u6027\u5730\u7406\u89e3\u76f8\u5173\u751f\u7269\u8fc7\u7a0b\u81f3\u5173\u91cd\u8981\u3002\u4f20\u7edf\u7684PPI\u7814\u7a76\u65b9\u6cd5\u5982\u4eb2\u548c\u8d28\u8c31\uff0c\u9700\u8981\u88c2\u89e3\u7ec6\u80de\uff0c\u4ece\u800c\u5206\u79bb\u548c\u9274\u5b9a\u4e92\u4f5c\u86cb\u767d\u3002\u7136\u800c\u88c2\u89e3\u7ec6\u80de\u4f1a\u5e72\u6270\u86cb\u767d\u7684\u4e9a\u7ec6\u80de\u5206\u5e03\u5e76\u7a00\u91ca\u86cb\u767d\uff0c\u53ef\u80fd\u4f1a\u5bfc\u81f4\u4e00\u4e9b\u7ed3\u5408\u86cb\u767d\u5206\u79bb\u6216\u8005\u4ea7\u751f\u4e00\u4e9b\u975e\u5929\u7136\u7684\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u3002\u800c\u57fa\u56e0\u7f16\u7801\u7684\u5177\u6709\u6f5c\u5728\u751f\u7269\u53cd\u5e94\u6027\u7684\u975e\u5929\u7136\u6c28\u57fa\u9178\uff08Uaa\uff09\u80fd\u591f\u539f\u4f4d\u6355\u83b7\u6d3b\u7ec6\u80de\u4e2d\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\u7684\u86cb\u767d\u8d28\uff0c\u5e76\u4e14\u901a\u8fc7\u9274\u5b9aUaa\u4ecb\u5bfc\u7684\u4ea4\u8054\u80bd\u6bb5\u6765\u786e\u5b9a\u76f8\u4e92\u4f5c\u7528\u754c\u9762\u3002\u4f46\u662f\u7531\u4e8e\u86cb\u767d\u6837\u54c1\u7684\u590d\u6742\u6027\u4ee5\u53ca\u4ea4\u8054\u80bd\u6bb5\u8f83\u4f4e\u7684\u79bb\u5b50\u5316\u6548\u7387\uff0c\u9274\u5b9aUaa\u4ecb\u5bfc\u7684\u4ea4\u8054\u80bd\u6bb5\u4ecd\u65e7\u5f88\u6709\u6311\u6218\u6027\u3002\u5373\u4f7f\u80bd\u6bb5\u6c34\u5e73\u5bcc\u96c6\u663e\u8457\u589e\u52a0\u4e86\u68c0\u6d4b\u7684\u7075\u654f\u5ea6\uff0c\u4f46\u662f\u4ecd\u5b58\u5728\u4ea4\u8054\u6548\u7387\u8f83\u4f4e\u7684\u95ee\u9898\uff0c\u5bfc\u81f4\u88ab\u9274\u5b9a\u7684\u4ea4\u8054\u80bd\u6570\u91cf\u8f83\u5c11\u3002\u540e\u6765\uff0c\u4eba\u4eec\u5f00\u53d1\u51fa\u4e86\u53ef\u5bcc\u96c6\u7684\u5177\u6709\u6f5c\u5728\u751f\u7269\u53cd\u5e94\u6027\u7684Uaa EB3\uff0c\u5e76\u5728\u6d3b\u7ec6\u80de\u6a21\u578b\u86cb\u767d\u4e2d\u6210\u529f\u9a8c\u8bc1\uff0c\u4f46\u662f\u8fd9\u79cdUaa\u53ea\u80fd\u4e0e\u86cb\u767d\u7ec4\u4e2d\u4e30\u5ea6\u8f83\u4f4e\u7684\u534a\u80f1\u6c28\u9178\u53cd\u5e94\uff0c\u4ecd\u7136\u65e0\u6cd5\u5f88\u597d\u5730\u6ee1\u8db3\u9700\u6c42\u3002\u6700\u7ec8\uff0c\u5177\u6709\u591a\u79cd\u6c28\u57fa\u9178\u53cd\u5e94\u6027\u3001\u4ea4\u8054\u6548\u7387\u8f83\u9ad8\u5e76\u4e14\u53ef\u5bcc\u96c6\u7684\u6c1f\u786b\u9178\u76d0-L-\u916a\u6c28\u9178\uff08FSY\uff09\u88ab\u5f00\u53d1\u51fa\u6765\uff0c\u8fd9\u79cdUaa\u5bf9Lys\u3001His\u4ee5\u53ca<span class=\"wx_search_keyword_wrap\">Tyr<\/span>\u4fa7\u94fe\u57fa\u56e2\u90fd\u5177\u6709\u53cd\u5e94\u6027\uff0c\u5728\u4f53\u5916\u548c\u6d3b\u7ec6\u80de\u4e2d\u90fd\u5177\u6709\u5f88\u9ad8\u7684\u4ea4\u8054\u6548\u7387\uff0c\u4f46\u662fFSY\u7684<span class=\"wx_search_keyword_wrap\">\u8131\u9776\u6548\u5e94<\/span>\u548c\u4ea4\u8054\u4ea7\u7269\u8fd8\u6ca1\u6709\u7ecf\u8fc7\u8d28\u8c31\u9a8c\u8bc1\u3002<\/p>\n<p>\u57fa\u4e8e\u4e0a\u8ff0\u80cc\u666f\uff0c\u4f5c\u8005\u5728FSY\u7684\u57fa\u7840\u4e0a\uff0c\u7ed3\u5408\u4e86MS\u53ef\u88c2\u89e3\u5c0f\u5206\u5b50\u4ea4\u8054\u5242\u4ee5\u53ca\u5316\u5b66\u88c2\u89e3\u4ea4\u8054Uaa\u7684\u7279\u70b9\uff0c\u8bbe\u8ba1\u51fa\u4e86\u4e00\u79cd\u4ece\u672a\u88ab\u62a5\u9053\u8fc7\u7684MS\u53ef\u88c2\u89e3\u5316\u5b66\u4ea4\u8054Uaa\uff08MSCXUaa\uff09\u3002MSCXUaa\u5177\u6709\u4ee5\u4e0b\u4f18\u70b9\uff1a\u9996\u5148\uff0c\u4e0e\u5c0f\u5206\u5b50\u4ea4\u8054\u5242\u76f8\u6bd4\uff0cMSCXUaa\u53ef\u4ee5\u8fdb\u5165\u6d3b\u7ec6\u80de\u4e2d\u5728\u751f\u7406\u6761\u4ef6\u4e0b\u539f\u4f4d\u4ea4\u8054\uff0c\u800c\u4e0d\u7528\u6740\u6b7b\u7ec6\u80de\uff0c\u80fd\u591f\u907f\u514d\u5bf9\u539f\u6709\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684\u5e72\u6270\u3002\u5176\u6b21\uff0c\u76f8\u8f83\u4e8e\u5316\u5b66\u88c2\u89e3\u7684\u5149\u4ea4\u8054Uaa\uff0cMSCXUaa\u80fd\u591f\u5728\u4e00\u7ea7\u8d28\u8c31\u4e2d\u4fdd\u6301\u4ea4\u8054\u80bd\u6bb5\u7684\u5b8c\u6574\u6027\uff0c\u589e\u52a0\u4ea4\u8054\u80bd\u6bb5\u9274\u5b9a\u7684\u7279\u5f02\u6027\u3002\u6700\u540e\uff0c\u8d28\u8c31\u53ef\u88c2\u89e3\u7684\u4ea4\u8054\u80bd\u6bb5\u76f8\u8f83\u4e8e\u4e0d\u53ef\u88c2\u89e3\u7684\u4ea4\u8054\u80bd\u6bb5\u5177\u6709\u66f4\u9ad8\u7684\u79bb\u5b50\u5316\u6548\u7387\uff0c\u53ef\u4ee5\u5728\u4e8c\u7ea7\u8d28\u8c31\u4e2d\u4ea7\u751f\u66f4\u591a\u5305\u542b\u4ea4\u8054\u4f4d\u70b9\u7684\u788e\u7247\u79bb\u5b50\uff0c\u8fdb\u800c\u63d0\u9ad8\u4ea4\u8054\u80bd\u6bb5\u7684\u788e\u7247\u79bb\u5b50\u8986\u76d6\u7387\u3002<\/p>\n<p>\u4f5c\u8005\u9996\u5148\u5c1d\u8bd5\u901a\u8fc7\u9057\u4f20\u5bc6\u7801\u5b50\u62d3\u5c55\u6280\u672f\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u8868\u8fbe\u57fa\u56e0\u7f16\u7801\u7684MSCXUaa\u3002\u4f5c\u8005\u901a\u8fc7\u4e94\u6b65\u5408\u6210\u5f97\u5230\u4e86MSCXUaa\u5e76\u547d\u540d\u4e3aeFSY\uff08\u5982\u56fe1a\u6240\u793a\uff09\uff0c\u5e76\u5c06\u6c28\u9170tRNA\u5408\u6210\u9176chPheRS-1\u7684\u6c28\u57fa\u9178\u7ed3\u5408\u53e3\u888b\u8fdb\u884c\u4e86\u6539\u9020\u4ee5\u5339\u914deFSY\u7684\u7ed3\u5408\u3002\u4f5c\u8005\u5c06190\u4f4d\u542b\u6709TAG\u5bc6\u7801\u5b50\u7684\u5e26\u6709His\u6807\u7b7e\u7684EGFP\u4e0echPheRS-1\u7684\u4e0d\u540c\u7a81\u53d8\u4f53\u5171\u8868\u8fbe\uff0c\u7136\u540e\u901a\u8fc7His\u6807\u7b7e\u7eaf\u5316\u7ed3\u5408\u51dd\u80f6\u7535\u6cf3\u4ee5\u53ca\u9ad8\u5206\u8fa8\u7535\u55b7\u96fe\u7535\u79bb<span class=\"wx_search_keyword_wrap\">\u98de\u884c\u65f6\u95f4\u8d28\u8c31<\/span>\u5206\u6790\u7b5b\u9009\u51fa\u4e86\u80fd\u591f\u8bc6\u522beFSY\u7684\u7a81\u53d8\u4f53\uff08V393G\uff0cF464C\uff09\uff0c\u5e76\u547d\u540d\u4e3aeFSYRS\u3002\u4e3a\u4e86\u9a8c\u8bc1eFSY\u7684\u6210\u529f\u63d2\u5165\uff0c\u4f5c\u8005\u5c0624\u4f4d\u4e3aTAG\u5bc6\u7801\u5b50\u7684\u5e26\u6709MBP\u4e0eHis\u6807\u7b7e\u7684Z\u86cb\u767d\u4e0eeFSYRS\u5728DH10B\u4e2d\u5171\u8868\u8fbe\uff0c\u5e76\u901a\u8fc7\u5b8c\u6574\u86cb\u767d\u8d28\u8c31\u4ee5\u53caGlu-C\u9176\u5207\u80bd\u6bb5\u4e32\u7ea7\u8d28\u8c31\u5206\u6790\u8bc1\u5b9eeFSY\u6210\u529f\u63d2\u5165\u5230\u4e86MBP-Z\u768424\u53f7\u4f4d\u70b9\uff08\u5982\u56fe1b-f\u6240\u793a\uff09\u3002<\/p>\n<p>\u5df2\u77e5FSY\u80fd\u591f\u4e0eHis\u3001Tyr\u4ee5\u53caLys\u4fa7\u94fe\u57fa\u56e2\u4ea4\u8054\uff0c\u4e3a\u4e86\u6d4b\u8bd5eFSY\u7684\u4ea4\u8054\u80fd\u529b\uff0c\u4f5c\u8005\u5c06MBP-Z\uff08E24eFSY\uff09\u4e0e\u4e0d\u540c\u4eb2\u548c\u7a81\u53d8\u4f53\uff087Ala\uff0c7Lys\uff0c7His\uff0c7Tyr\uff09\u5171\u5b75\u80b2\u3002WB\u548c\u4e32\u7ea7\u8d28\u8c31\u8bc1\u660e\u4e86eFSY\u80fd\u591f\u4e0eHis\u3001Tyr\u548cLys\u4ea7\u751f\u4ea4\u8054\u3002\u901a\u8fc7\u5728eFSY\u4f4d\u70b9\u5f15\u5165\u751f\u7269\u7d20\u6807\u7b7e\u5e76\u7528\u5355\u4eb2\u548c\u5fae\u7403\u8fdb\u884c\u5bcc\u96c6\uff0c\u4ea4\u8054\u80bd\u6bb5\u7684\u5bcc\u96c6\u5ea6\u589e\u52a0\u4e86\u5341\u500d\uff0c\u9a8c\u8bc1\u4e86eFSY\u53ef\u5bcc\u96c6\u7684\u7279\u70b9\uff08\u5982\u56fe1g-l\u6240\u793a\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"1\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240624\/1719197069281476.webp\" alt=\"1\" \/><\/section>\n<section>\n<section>\u56fe1. eFSY\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u7684\u8868\u8fbe\u4e0e\u9274\u5b9a\u793a\u610f\u56fe<\/p>\n<\/section>\n<\/section>\n<section>\n<section>\u63a5\u7740\uff0c\u4f5c\u8005\u5c1d\u8bd5\u7528eFSY\u6765\u786e\u5b9aTrx1\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u7684\u76f4\u63a5\u4f5c\u7528\u86cb\u767d\u3002\u4f5c\u8005\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u5c06eFSY\u5f15\u5165Trx1\u768462\u53f7\u4f4d\u70b9\uff08\u5982\u56fe2a\u6240\u793a\uff09\uff0c\u5e76\u4f7f\u7528BprY\u3001FSK\u4f5c\u4e3a\u5bf9\u7167\u3002WB\u7ed3\u679c\u663e\u793a\u51fa\u4f17\u591a\u53ef\u4ee5\u88ab\u751f\u7269\u7d20\u6807\u8bb0\u7684\u4ea4\u8054\u6761\u5e26\uff0c\u8868\u660eeFSY\u53ef\u4ee5\u6210\u529f\u6355\u83b7\u5230Trx1\u7684\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\uff08\u5982\u56fe2b-c\u6240\u793a\uff09\u3002\u7ecf\u8fc7His\u6807\u7b7e\u7eaf\u5316\u3001\u751f\u7269\u7d20\u6807\u8bb0\u4ee5\u53ca\u94fe\u9709\u4eb2\u548c\u7d20\u5bcc\u96c6\u4e4b\u540e\uff0c\u5728\u4e09\u6b21\u91cd\u590d\u5b9e\u9a8c\u4e2d\u5171\u9274\u5b9a\u5230150\u4e2a\u86cb\u767d\u4e0a\u7684311\u4e2a\u4ea4\u8054\u4f4d\u70b9\uff08\u5982\u56fe2d-e\u6240\u793a\uff09\u3002\u4e0a\u8ff0\u7ed3\u679c\u8868\u660eeFSY\u5177\u6709\u8f83\u9ad8\u7684\u86cb\u767d\u4ea4\u8054\u6548\u7387\u3002<\/p>\n<p>\u4f5c\u8005\u5728\u5206\u6790\u8d28\u8c31\u6570\u636e\u65f6\u53d1\u73b0eFSY\u4e0eHis\u3001Lys\u4ea7\u751f\u7684\u4ea4\u8054\u662f\u8d28\u8c31\u53ef\u88c2\u89e3\u7684\uff0c\u4e0eTyr\u4ea7\u751f\u7684\u4ea4\u8054\u662f\u8d28\u8c31\u4e0d\u53ef\u88c2\u89e3\u7684\uff0c\u800c\u5728\u53ef\u88c2\u89e3\u7684\u4ea4\u8054\u4e2d\u8fd8\u5305\u62ec\u4ea4\u8054\u952e\u65ad\u88c2\u4e0e\u4ea4\u8054\u57fa\u56e2\u5185\u90e8\u788e\u88c2\u4e24\u79cd\u788e\u88c2\u6a21\u5f0f\uff08\u5982\u56fe2f-i\uff0c3a-b\u6240\u793a\uff09\u3002\u7531\u4e8e\u76ee\u524d\u6ca1\u6709\u80fd\u591f\u5b8c\u7f8e\u5206\u6790\u8fd9\u79cd\u4ea4\u8054\u8d28\u8c31\u6570\u636e\u7684\u8f6f\u4ef6\uff0c\u4f5c\u8005\u5f00\u53d1\u51fa\u4e86\u4e00\u4e2a\u80fd\u591f\u8003\u8651\u5230\u6587\u4e2d\u6240\u6709\u5f62\u5f0f\u7684\u788e\u7247\u79bb\u5b50\u7684\u641c\u7d22\u5f15\u64ceAixUaa\u3002AixUaa\u8fd0\u884c\u7684\u57fa\u672c\u903b\u8f91\u5982\u56fe3c\u6240\u793a\u3002\u4e3a\u4e86\u8bc4\u4f30AixUaa\u9274\u5b9aUaa\u4ecb\u5bfc\u7684MS\u53ef\u88c2\u89e3\u7684\u4ea4\u8054\u8d28\u8c31\u6570\u636e\u7684\u80fd\u529b\uff0c\u4f5c\u8005\u6784\u5efa\u4e86\u56db\u4e2a\u6a21\u62df\u7684U-H\/K\u4ea4\u8054\u6570\u636e\u5e93Sim1-4\uff0c\u5e76\u5c06AixUaa\u4e0e\u73b0\u6709\u641c\u7d22\u5f15\u64ce\u8fdb\u884c\u5bf9\u6bd4\uff0c\u7ed3\u679c\u8868\u660e\u76f8\u8f83\u4e8e\u73b0\u6709\u7684\u6570\u636e\u5e93\u641c\u7d22\u5f15\u64ce\uff0cAixUaa\u5c55\u793a\u51fa\u4e86\u66f4\u9ad8\u7684\u9274\u5b9a\u7cbe\u5ea6\u3002\u4f5c\u8005\u53c8\u5c06AixUaa\u7528\u4e8e\u5206\u6790\u786b\u6c27\u5316\u8fd8\u539f\u86cb\u767d\u5bcc\u96c6\u6837\u54c1\uff0c\u5982\u56fe3d-e\u6240\u793a\uff0cAixUaa\u9274\u5b9a\u51fa\u4e86\u66f4\u591a\u7684U-H\/K\u4ea4\u8054\u4f4d\u70b9\uff0c\u603b\u7684\u4ea4\u8054\u80bd\u6bb5\u9274\u5b9a\u6570\u4e3a391\uff0c\u5bf9\u5e94184\u4e2a\u86cb\u767d\u3002\u4ece\u56fe3f\u4e2d\u53ef\u4ee5\u770b\u51fa\uff0c\u9274\u5b9a\u5230\u7684\u4ea4\u8054\u4f4d\u70b9\u4e3b\u8981\u4e3aLys\uff0c\u8fd9\u53ef\u80fd\u4e0eLys\u5728\u7ec6\u80de\u4e2d\u7684\u8f83\u9ad8\u4e30\u5ea6\u6709\u5173\u3002<\/p>\n<\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"2\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240624\/1719197093937545.webp\" alt=\"2\" \/><\/section>\n<section>\n<section>\u56fe2. \u5728\u5927\u80a0\u6746\u83cc\u4e2d\u7528eFSY\u63d2\u5165\u7684Trx1\u6355\u83b7\u9274\u5b9aTrx1\u7684\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u7ec4<\/p>\n<\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"3\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240624\/1719197104994753.webp\" alt=\"3\" \/><\/section>\n<section>\n<section>\u56fe3. eFSY\u4ea4\u8054\u80bd\u6bb5\u7684\u788e\u88c2\u6a21\u5f0f\u4e0eAixUaa\u7a0b\u5e8f\u7684\u5f00\u53d1<\/p>\n<\/section>\n<\/section>\n<section><\/section>\n<section>\n<section>\u4f5c\u8005\u63a5\u7740\u6d4b\u8bd5\u4e86\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2deFSY\u7684\u63d2\u5165\u548c\u4ea4\u8054\u80fd\u529b\uff0c\u5728HEK293T\u7ec6\u80de\u4e2d\u5171\u8f6c\u67d3EGFP-151TAG\u4ee5\u53capNEU-eFSYRS\u8d28\u7c92\uff0c\u5e76\u5c06\u7ec6\u80de\u7f6e\u4e8e\u542b\u6709eFSY\u7684\u57f9\u517b\u57fa\u4e2d\u57f9\u517b\u3002\u8367\u5149\u663e\u5fae\u955c\u89c2\u5bdf\u5f97\u5230\u7684\u8367\u5149\u4fe1\u53f7\u4ee5\u53ca\u5b8c\u6574\u86cb\u767d\u8d28\u8c31\u548c\u4e32\u7ea7\u8d28\u8c31\u6570\u636e\u8bc1\u660e\u4e86EGFP-190eFSY\u7684\u6210\u529f\u8868\u8fbe\uff08\u5982\u56fe4a-c\u6240\u793a\uff09\u3002\u4e3a\u4e86\u786e\u5b9aeFSY\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u7684\u4ea4\u8054\u80fd\u529b\uff0c\u4f5c\u8005\u5728HEK293T\u7ec6\u80de\u7684\u8c37\u80f1\u7518\u80bdS-\u8f6c\u79fb\u9176(GST)\u7684\u4e8c\u805a\u4f53\u754c\u9762103\u4f4d\u70b9\u5f15\u5165\u4e86eFSY\u3002WB\u6210\u529f\u68c0\u6d4b\u5230\u4e86eFSY\u4ecb\u5bfc\u7684\u4e8c\u805a\u4f53\u4ea4\u8054\u5e26(\u5982\u56fe4d\u6240\u793a)\u3002\u8fdb\u4e00\u6b65\u7684\u4e32\u8054\u8d28\u8c31\u5206\u6790\u9274\u5b9a\u51fa\u4e09\u4e2a\u94fe\u95f4\u4ea4\u8054\u80bd\uff0c\u4ea4\u8054\u4f4d\u70b9\u4e0eeFSY\u7ed3\u5408\u4f4d\u70b9\u975e\u5e38\u63a5\u8fd1(\u5982\u56fe4e\u6240\u793a)\uff0c\u5e76\u4e14\u8fd9\u4e9b\u4ea4\u8054\u4f4d\u70b9\u80fd\u591f\u901a\u8fc7\u9ad8\u8d28\u91cf\u4e32\u8054\u8d28\u8c31\u7cbe\u786e\u5b9a\u4f4d(\u5982\u56fe4f-h\u6240\u793a)\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0ceFSY\u53ef\u4ee5\u6709\u6548\u5730\u63ba\u5165\u5230\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\uff0c\u5e76\u4e14\u5728\u6d3b\u7ec6\u80de\u4e2d\u5177\u6709\u8f83\u9ad8\u7684\u4ea4\u8054\u6548\u7387\u3002<\/p>\n<\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"4\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240624\/1719197114484019.webp\" alt=\"4\" \/><\/section>\n<section>\n<section>\u56fe4. eFSY\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u8868\u8fbe\u5e76\u7528\u4e8e\u4ea4\u8054\u9274\u5b9a\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u7ec4<\/p>\n<\/section>\n<\/section>\n<section><\/section>\n<section>\n<section>\u5fae\u91cf\u5143\u7d20\u7852\u5728\u7ec6\u80de\u4e2d\u4ee5\u7852\u4ee3\u534a\u80f1\u6c28\u9178\u7684\u5f62\u5f0f\u5b58\u5728\u4e8e\u7852\u86cb\u767d\u7684\u6d3b\u6027\u4f4d\u70b9\uff0c\u7136\u800c\u7531\u4e8e\u7852\u4ee3\u534a\u80f1\u6c28\u9178\u662f\u7531\u7ec8\u6b62\u5bc6\u7801\u5b50<span class=\"wx_search_keyword_wrap\">TGA\u7f16\u7801<\/span>\u7684\uff0c\u96be\u4ee5\u83b7\u5f97\u5927\u91cf\u5929\u7136\u7684\u7852\u86cb\u767d\uff0c\u7852\u86cb\u767d\u7684\u529f\u80fd\u8fd8\u6ca1\u6709\u5f97\u5230\u6df1\u5165\u7684\u7814\u7a76\u3002\u56e0\u6b64\uff0c\u4f5c\u8005\u5c06eFSY\u7528\u4e8e\u68c0\u6d4b\u7852\u86cb\u767d\u7684\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u7ec4\uff0c\u8fdb\u800c\u66f4\u597d\u5730\u4e86\u89e3\u7852\u86cb\u767d\u7684\u529f\u80fd\u3002\u4f5c\u8005\u5c06eFSY\u63d2\u5165\u5230\u6d3b\u7ec6\u80de\u4e2d\u7852\u86cb\u767dSELM\u768448\u53f7\u7852\u4ee3\u534a\u80f1\u6c28\u9178\u4f4d\u70b9\u4e0a\uff08\u5982\u56fe5a\u6240\u793a\uff09\uff0c\u9996\u5148\u8fdb\u884c\u4e86WB\u5206\u6790\uff0c\u7ed3\u679c\u663e\u793aSELM\u4e0e\u5185\u6e90\u86cb\u767d\u53d1\u751f\u4e86\u4ea4\u8054\uff0c\u5e76\u4e14\u8fd9\u4e9b\u4ea4\u8054\u86cb\u767d\u590d\u5408\u4f53\u80fd\u591f\u88ab\u751f\u7269\u7d20\u6807\u8bb0\uff08\u5982\u56fe5b-c\u6240\u793a\uff09\u3002\u63a5\u7740\u4f5c\u8005\u5c06\u4ea4\u8054\u86cb\u767d\u7528Strep IP\u7eaf\u5316\u540e\uff0c\u7528\u751f\u7269\u7d20\u6807\u8bb0\uff0c\u63a5\u7740\u7528\u80f0\u9176\u6c34\u89e3\uff0c\u518d\u7528\u5355\u4eb2\u548c\u51dd\u73e0\u5bcc\u96c6\u4ea4\u8054\u80bd\u6bb5\uff0c\u6700\u540e\u8fdb\u884c\u8d28\u8c31\u5206\u6790\u3002\u5728\u4e09\u6b21\u91cd\u590d\u8bd5\u9a8c\u4e2d\u5171\u9274\u5b9a\u523024\u4e2a\u4ea4\u8054\u80bd\u6bb5\uff0c\u5bf9\u5e9410\u4e2a\u4e0eSELM\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\u7684\u86cb\u767d\uff08\u5982\u56fe5d\u6240\u793a\uff09\u3002SELM\u5b9a\u4f4d\u4f4d\u4e8e\u6838\u5468\u7ed3\u6784\u4e2d\uff0c\u5bf9\u5e94\u4e8e\u5185\u8d28\u7f51\u548c\u9ad8\u5c14\u57fa\u4f53\uff0c\u800c\u9274\u5b9a\u5f97\u5230\u7684\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u540c\u6837\u4e3b\u8981\u5206\u5e03\u4e8e\u5185\u8d28\u7f51\u548c\u9ad8\u5c14\u57fa\u4f53\uff0c\u8fd9\u8bc1\u660e\u4e86eFSY\u4ea4\u8054\u5177\u6709\u4e00\u5b9a\u7684\u7a7a\u95f4\u7279\u5f02\u6027\u3002\u4f5c\u8005\u5728\u8fd9\u4e9b\u86cb\u767d\u4e2d\u968f\u673a\u9009\u62e9\u4e86\u56db\u4e2a\u86cb\u767d\u8fdb\u884cCo-IP\u5b9e\u9a8c\uff0cWB\u5206\u6790\u7ed3\u679c\u8bc1\u5b9e\u4e86\u8fd9\u4e9b\u86cb\u767d\u786e\u5b9e\u4e0eSELM\u5b58\u5728\u76f8\u4e92\u4f5c\u7528\uff08\u5982\u56fe5e\u6240\u793a\uff09\u3002\u63a5\u7740\uff0c\u4f5c\u8005\u9009\u62e9\u4ea4\u8054\u4f4d\u70b9\u9644\u8fd1\u542b\u6709\u534a\u80f1\u6c28\u9178\u7684PRDX4\u86cb\u767d\uff0c\u4f7f\u7528\u4e8c\u786b\u952e\u6355\u83b7\u6cd5\u518d\u6b21\u8bc1\u5b9e\u4e86SELM\u4e0ePRDX4\u4e4b\u95f4\u5b58\u5728\u76f8\u4e92\u4f5c\u7528\uff08\u5982\u56fe5f-h\u6240\u793a\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"5\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240624\/1719197129465601.webp\" alt=\"5\" \/>\u200b<\/section>\n<section>\n<section>\u56fe5. \u901a\u8fc7eFSY\u4ecb\u5bfc\u7684\u539f\u4f4d\u4ea4\u8054\u9274\u5b9aSELM\u7684\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u7ec4<\/p>\n<\/section>\n<\/section>\n<section>\n<section>\u7efc\u4e0a\u6240\u8ff0\uff0c\u672c\u6587\u4e2d\u4f5c\u8005\u8bbe\u8ba1\u5e76\u5408\u6210\u4e86\u4e00\u79cd\u5177\u6709\u6f5c\u5728\u751f\u7269\u53cd\u5e94\u6027\u3001\u53ef\u5bcc\u96c6\u6027\u5e76\u4e14MS\u53ef\u88c2\u89e3\u7684Uaa &#8211; eFSY\uff0c\u540c\u65f6\u901a\u8fc7\u5b9a\u5411\u8fdb\u5316\u83b7\u5f97\u4e86\u4e00\u79cd\u80fd\u9ad8\u6548\u8bc6\u522beFSY\u7684\u6c28\u57fa\u9170\u57fa- tRNA\u5408\u6210\u9176eFSYRS\u3002\u5229\u7528eFSYRS\uff0c\u4f5c\u8005\u5728\u5927\u80a0\u6746\u83cc\u548c\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u6210\u529f\u63d2\u5165\u4e86eFSY\uff0c\u800c\u63d2\u5165\u7684eFSY\u6210\u529f\u5730\u6355\u83b7\u4e86\u77ac\u65f6\u548c\u76f4\u63a5\u7684\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u4f4d\u70b9\u3002eFSY\u4e0a\u5e26\u6709\u7684click\u6293\u624b\u8fd8\u53ef\u4ee5\u8fdb\u884c\u591a\u80bd\u6c34\u5e73\u5bcc\u96c6\uff0c\u8fdb\u800c\u5b9e\u73b0\u5927\u80a0\u6746\u83cc\u548c\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2dPPIs\u7684\u9ad8\u901a\u91cf\u9274\u5b9a\u3002\u57fa\u4e8eeFSY\u4ea4\u8054\u80bd\u6bb5\u7684\u8d28\u8c31\u88c2\u89e3\u6a21\u5f0f\uff0c\u4f5c\u8005\u8fd8\u5f00\u53d1\u4e86\u641c\u7d22\u5f15\u64ceAixUaa\uff0c\u4ee5\u63d0\u9ad8\u8d28\u8c31\u9274\u5b9a\u7684\u7075\u654f\u5ea6\u548c\u5e8f\u5217\u8986\u76d6\u7387\u3002<\/p>\n<\/section>\n<\/section>\n<section>\u672c\u6587\u4f5c\u8005\uff1aLHC<\/p>\n<p>\u539f\u6587\u5f15\u7528\uff1a<\/p>\n<p>https:\/\/doi.org\/10.1038\/s41467-024-49517-1<\/p>\n<p>\u8d23\u4efb\u7f16\u8f91\uff1aLD<\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u63a8\u8350\u4e00\u7bc7\u53d1\u8868\u5728Nature Communication\u4e0a\u7684\u6587\u7ae0\uff0c\u6587\u7ae0\u6807\u9898\u4e3a\u201cCharacterize dire [&hellip;]<\/p>\n","protected":false},"author":326,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-51762","post","type-post","status-publish","format-standard","hentry","category-tuijianyuedu"],"_links":{"self":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51762","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/users\/326"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/comments?post=51762"}],"version-history":[{"count":1,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51762\/revisions"}],"predecessor-version":[{"id":51763,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51762\/revisions\/51763"}],"wp:attachment":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/media?parent=51762"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/categories?post=51762"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/tags?post=51762"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}