{"id":51602,"date":"2024-06-09T08:05:02","date_gmt":"2024-06-09T00:05:02","guid":{"rendered":"https:\/\/www.chemhui.com\/?p=51602"},"modified":"2024-05-31T10:05:32","modified_gmt":"2024-05-31T02:05:32","slug":"chembiochem%ef%bc%9a%e5%9f%ba%e4%ba%8e%e9%ab%98%e9%80%9a%e9%87%8f%e8%b4%a8%e8%b0%b1%e5%92%8c%e4%bd%93%e5%86%85-%e4%bd%93%e5%a4%96%e9%85%b6%e5%82%ac%e5%8c%96%e6%80%a7%e8%83%bd%e5%85%b3%e8%81%94","status":"publish","type":"post","link":"http:\/\/www.chemhui.com\/chembiochem%ef%bc%9a%e5%9f%ba%e4%ba%8e%e9%ab%98%e9%80%9a%e9%87%8f%e8%b4%a8%e8%b0%b1%e5%92%8c%e4%bd%93%e5%86%85-%e4%bd%93%e5%a4%96%e9%85%b6%e5%82%ac%e5%8c%96%e6%80%a7%e8%83%bd%e5%85%b3%e8%81%94\/","title":{"rendered":"ChemBioChem\uff1a\u57fa\u4e8e\u9ad8\u901a\u91cf\u8d28\u8c31\u548c\u4f53\u5185\/\u4f53\u5916\u9176\u50ac\u5316\u6027\u80fd\u5173\u8054\u7684\u86cb\u767d\u8d28\u5de5\u7a0b"},"content":{"rendered":"<section>\n<section>\n<section>\n<section>\n<section>\u4e09\u4e59\u9178\u5185\u916f\uff08TAL\uff09\u662f\u751f\u7269\u5de5\u7a0b\u7684\u91cd\u8981\u4ea7\u54c1\uff08\u56fe1A\uff09\uff0c\u5b83\u662f\u8bf8\u591a\u5546\u4e1a\u5316\u5b66\u54c1\u548c\u836f\u7269\u7684\u5173\u952e\u524d\u4f53\u3002\u5728\u4ee3\u8c22\u5de5\u7a0b\u9886\u57df\uff0cTAL\u7684\u4ea7\u91cf\u5df2\u7ecf\u83b7\u5f97\u5f88\u5927\u63d0\u5347\uff0c\u4f46\u50ac\u5316TAL\u751f\u6210\u7684\u5173\u952e\u91762-\u5421\u5583\u916e\u5408\u9176\uff082PS\uff0c\u56fe1B\uff09\u5bf9\u5e95\u7269\u4e19\u4e8c\u9170\u8f85\u9176A\uff08malonyl-CoA\uff09\u7684\u50ac\u5316\u6548\u7387\u4ec5\u6709\u5927\u7ea610<sup>2<\/sup>\u00a0M<sup>-1<\/sup>s<sup>-1<\/sup>\uff0c\u8fdc\u4f4e\u4e8e\u5176\u4ed6\u6b21\u751f\u4ee3\u8c22\u9176\u7684\u5e73\u5747\u6027\u80fd\u3002\u4ee5\u5f80\u6587\u732e\u62a5\u9053\u4e2d\u4e5f\u6709\u901a\u8fc7\u86cb\u767d\u8d28\u5de5\u7a0b\u6539\u90202PS\u7684\u5de5\u4f5c\uff0c\u4f46\u4ecd\u7f3a\u4e4f\u7cfb\u7edf\u6027\uff0c\u6d3b\u6027\u4f4d\u70b9\u5468\u56f4\u8fd8\u6709\u5f88\u5927\u6539\u8fdb\u7a7a\u95f4\u3002\u5b9a\u5411\u8fdb\u5316\u662f\u8f83\u597d\u7684\u7814\u7a76\u65b9\u6848\uff0c\u4f46\u7f3a\u4e4f\u9ad8\u6548\u7684\u65b9\u6cd5\u5bf9\u5927\u91cf\u6539\u9020\u83cc\u682a\u7684\u4ea7\u91cf\u8fdb\u884c\u7cbe\u51c6\u68c0\u6d4b\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p>&nbsp;<\/p>\n<section>\n<section><\/section>\n<\/section>\n<p>&nbsp;<\/p>\n<section><img decoding=\"async\" title=\"1\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20240422\/1713755161544339.webp\" alt=\"1\" \/><\/section>\n<section>\u56fe1: A) TAL\u53ca\u5176\u8f6c\u5316\u7269\u7684\u5e94\u7528-\u84dd\u8272\u6807\u8bb0\u7684\u5206\u5b50\u53ef\u7528\u4f5c\u98df\u54c1\u6dfb\u52a0\u5242\u3001\u82b3\u9999\u5242\u7b49\uff0c\u7ea2\u8272\u6807\u8bb0\u7684\u5206\u5b50\u53ef\u7528\u4f5c\u57fa\u7840\u5408\u6210\u539f\u6599\uff1bB) 2PS\u6676\u4f53\u7ed3\u6784\uff1bC)\u00a0\u6210\u50cf\u8d28\u8c31\u9ad8\u901a\u91cf\u5b9a\u91cf\u6d41\u7a0b<\/p>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\u4e3a\u8fdb\u4e00\u6b65\u4f18\u53162PS\u6548\u80fd\uff0c\u5fb7\u514b\u8428\u65af\u5927\u5b66\u5965\u65af\u6c40\u5206\u6821Lu Yi\u6559\u6388\u56e2\u961f\u4e0e\u4f0a\u5229\u8bfa\u4f0a\u5927\u5b66\u9999\u69df\u5206\u6821Jonathan V. Sweedler\u6559\u6388\u5408\u4f5c\uff0c\u5728\u4e4b\u524d\u5bf92PS\u86cb\u767d\u8d28\u5de5\u7a0b\u7684\u7814\u7a76\u57fa\u7840\u4e0a\u00a0(<em>Angew. Chem. Int. Ed., DOI: 10.1002\/anie.202212440<\/em>)\uff0c\u57fa\u4e8e\u4f53\u5185\/\u4f53\u5916\u9176\u50ac\u5316\u6d3b\u6027\u5173\u8054\u5bf92PS\u8fdb\u884c\u4e86\u7cfb\u7edf\u6027\u7684\u86cb\u767d\u8d28\u5de5\u7a0b\u6539\u9020\uff0c\u5e76\u91c7\u7528\u9ad8\u901a\u91cf\u6210\u50cf\u8d28\u8c31\u65b9\u6cd5\uff08\u56fe1C\uff0c<em>Anal. Chem., DOI: 10.1021\/acs.analchem.2c04845<\/em>\uff09\u5bf9TAL\u7684\u4ea7\u91cf\u8fdb\u884c\u5feb\u901f\u5b9a\u91cf\uff081\u5206\u949f\/\u6837\u54c1\uff09\u3002<\/p>\n<p>\u901a\u8fc7\u591a\u8f6e\u5b9a\u5411\u8fdb\u5316\uff0c\u56e2\u961f\u6210\u529f\u83b7\u5f97\u4f18\u8d282PS\u6539\u9020\u9176\u3002\u5176\u4e2dL202G\/G205S\/M259L\/L261C-2PS\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u7684TAL\u4ea7\u91cf\u6bd4\u91ce\u751f\u578b\u9ad846\u500d\u3001\u6bd4\u62a5\u9053\u8fc7\u7684\u6700\u4f732PS\u7a81\u53d8\u578b\uff08L202G\/L261N-2PS\uff09\u9ad82.5\u500d\uff1b\u53e6\u4e00\u7a81\u53d8\u578bL202G\/L261C-2PS\u7684\u50ac\u5316\u6548\u7387(k<sub>cat<\/sub>)\u6bd4\u91ce\u751f\u578b\u9ad844\u500d\uff0c\u6bd4L202G\/L261N-2PS\u9ad81.5\u500d\uff08\u56fe2A\uff09\u3002<\/p>\n<p>\u901a\u8fc7\u6d4b\u5b9a\u7c73\u6c0f\u5e38\u6570\uff0c\u4f5c\u8005\u53d1\u73b0L202G\/L261C-2PS\u7684\u50ac\u5316\u6548\u7387\u867d\u7136\u6700\u9ad8\uff0c\u4f46\u5bf9\u5e95\u7269malonyl-CoA\u7684\u4eb2\u548c\u529b\u4f4e\u3002\u76f8\u6bd4\u4e8eL202G\/G205S\/M259L\/L261C-2PS\uff0c \u5b83\u7684\u4f4e\u4ea7\u91cf\u8868\u660e\u4e86\u9176\u548c\u5e95\u7269\u4eb2\u548c\u529b\u5bf9\u4ee3\u8c22\u53cd\u5e94\u7684\u5f71\u54cd\u3002\u4f5c\u8005\u5229\u7528\u5408\u6210\u751f\u7269\u5b66\u8f6c\u5316\u4e86\u4e00\u6761\u989d\u5916\u7684\u4ee3\u8c22\u9014\u5f84\uff0c\u4f7f\u5927\u80a0\u6746\u83cc\u80fd\u591f\u901a\u8fc7<span class=\"wx_search_keyword_wrap\">\u4e19\u4e8c\u9178<\/span>\u751f\u6210\u66f4\u591a\u7684malonyl-CoA\u3002\u4f5c\u8005\u60ca\u5947\u5730\u53d1\u73b0\uff0c\u5f53malonyl-CoA\u6d53\u5ea6\u63d0\u9ad8\u7ea6\u4e09\u500d\u65f6\uff0c\u6700\u4f18\u4ea7\u91cf\u7684\u7a81\u53d8\u578b\u4eceL202G\/G205S\/M259L\/L261C-2PS\u53d8\u6210\u4e86\u62e5\u6709\u66f4\u5feb\u53cd\u5e94\u901f\u7387\u7684L202G\/L261C-2PS\u3002\u8fd9\u4e00\u73b0\u8c61\u663e\u8457\u5730\u5c55\u73b0\u4e86\u4f53\u5185\/\u4f53\u5916\u7684\u86cb\u767d\u8d28\u5de5\u7a0b\u7684\u5dee\u5f02\uff0c\u5e76\u4e14\u5f3a\u8c03\u4e86\u6b63\u786e\u7684\u9009\u62e9\u538b\u529b\u7684\u91cd\u8981\u6027\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u8fd9\u79cd\u7ec6\u80de\u5185\u7684\u9009\u62e9\u538b\u529b\u901a\u5e38\u5f88\u96be\u901a\u8fc7\u4f53\u5916\u5b9e\u9a8c\u73af\u5883\u53bb\u6a21\u62df\uff0c\u56e0\u4e3a\u50cfmalonyl-CoA\u8fd9\u6837\u7684\u4e2d\u5fc3\u4ee3\u8c22\u7269\u5728\u4f53\u5185\u4f1a\u6301\u7eed\u751f\u6210\u3001\u9ad8\u5ea6\u8c03\u63a7\u5e76\u4e14\u7ef4\u6301\u5728\u7279\u5b9a\u7684\u6d53\u5ea6\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section><img decoding=\"async\" title=\"7\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20240422\/1713755180244210.webp\" alt=\"7\" \/><\/section>\n<section>\u56fe2\uff1aA\uff09\u5173\u952e\u53d8\u79cd\u7684\u4ea7\u91cf\u5bf9\u6bd4\uff1bB\uff09\u00a0\u5173\u952e\u7a81\u53d8\u578b\u7684\u7c73\u5f0f\u65b9\u7a0b\uff0cMatB\/C\u9014\u5f84\u7528\u4e8e\u5408\u6210malonyl-CoA\u3002<\/p>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\u672c\u6587\u4f5c\u8005\u5c06\u86cb\u767d\u5de5\u7a0b\u4e0e\u9ad8\u901a\u91cf\u7b5b\u9009\u76f8\u7ed3\u5408\uff0c\u7cfb\u7edf\u6027\u5730\u7814\u7a76\u5206\u6790\u4e86\u4f53\u5185\/\u4f53\u5916\u9176\u50ac\u5316\u6027\u80fd\u7684\u5dee\u5f02\uff0c\u5e76\u83b7\u5f97\u4e86\u4e24\u4e2a2PS\u7684\u9ad8\u6548\u7a81\u53d8\u4f53\uff0c\u8868\u73b0\u51fa44\u500d\u4ee5\u4e0a\u7684k<sub>cat<\/sub>\u300125\u500d\u4ee5\u4e0a\u7684\u50ac\u5316\u6548\u7387\u548c46\u500d\u4ee5\u4e0a\u7684TAL\u4ea7\u91cf\u3002\u8fd9\u4e00\u5de5\u4f5c\u6d41\u7a0b\u53ef\u4ee5\u5e94\u7528\u4e8e\u5176\u4ed6\u5929\u7136\u4ea7\u7269\u7684\u86cb\u767d\u8d28\u5de5\u7a0b\u548c\u5408\u6210\u751f\u7269\u5b66\u7684\u7814\u7a76\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section>\n<section>\n<section>\n<section><strong>\u8bba<\/strong><strong>\u6587\u4fe1\u606f<\/strong><\/p>\n<\/section>\n<\/section>\n<section>\n<section><\/section>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>Enhancing 2-Pyrone Synthase Efficiency by High-Throughput Mass-Spectrometric Quantification and In\u2005Vitro\/In\u2005Vivo Catalytic Performance Correlation<\/p>\n<p><span class=\"wx_search_keyword_wrap\">Yu Zhou<\/span>,\u00a0Shuaizhen Zhou,\u00a0Scott Lyons,\u00a0Haoran Sun,\u00a0Prof. Jonathan V. Sweedler,\u00a0Prof. Yi Lu<\/p>\n<p><em>ChemBioChem<\/em><\/p>\n<p>DOI:\u00a010.1002\/cbic.202300849<\/p>\n<\/section>\n<\/section>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u4e09\u4e59\u9178\u5185\u916f\uff08TAL\uff09\u662f\u751f\u7269\u5de5\u7a0b\u7684\u91cd\u8981\u4ea7\u54c1\uff08\u56fe1A\uff09\uff0c\u5b83\u662f\u8bf8\u591a\u5546\u4e1a\u5316\u5b66\u54c1\u548c\u836f\u7269\u7684\u5173\u952e\u524d\u4f53\u3002\u5728\u4ee3\u8c22\u5de5\u7a0b\u9886\u57df\uff0cTAL [&hellip;]<\/p>\n","protected":false},"author":326,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-51602","post","type-post","status-publish","format-standard","hentry","category-tuijianyuedu"],"_links":{"self":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51602","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/users\/326"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/comments?post=51602"}],"version-history":[{"count":1,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51602\/revisions"}],"predecessor-version":[{"id":51603,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51602\/revisions\/51603"}],"wp:attachment":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/media?parent=51602"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/categories?post=51602"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/tags?post=51602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}