{"id":51392,"date":"2024-05-08T08:36:59","date_gmt":"2024-05-08T00:36:59","guid":{"rendered":"https:\/\/www.chemhui.com\/?p=51392"},"modified":"2024-04-29T10:39:43","modified_gmt":"2024-04-29T02:39:43","slug":"jacs-%e5%9f%ba%e4%ba%8eusp7%e7%9a%84%e5%8e%bb%e6%b3%9b%e7%b4%a0%e5%8c%96%e9%85%b6%e9%9d%b6%e5%90%91%e5%b5%8c%e5%90%88%e4%bd%93%e7%a8%b3%e5%ae%9aampk","status":"publish","type":"post","link":"http:\/\/www.chemhui.com\/jacs-%e5%9f%ba%e4%ba%8eusp7%e7%9a%84%e5%8e%bb%e6%b3%9b%e7%b4%a0%e5%8c%96%e9%85%b6%e9%9d%b6%e5%90%91%e5%b5%8c%e5%90%88%e4%bd%93%e7%a8%b3%e5%ae%9aampk\/","title":{"rendered":"JACS |\u57fa\u4e8eUSP7\u7684\u53bb\u6cdb\u7d20\u5316\u9176\u9776\u5411\u5d4c\u5408\u4f53\u7a33\u5b9aAMPK"},"content":{"rendered":"<p>\u5206\u4eab\u4e00\u7bc7\u53d1\u8868\u5728JACS\u6742\u5fd7\u4e0a\u7684\u6587\u7ae0\uff0c\u9898\u76ee\u4e3a\u201cUSP7-Based Deubiquitinase-Targeting Chimeras Stabilize AMPK\u201d\u3002\u6587\u7ae0\u7684\u901a\u8baf\u4f5c\u8005\u662f\u897f\u5948\u5c71\u4f0a\u574e\u533b\u5b66\u9662\u7684\u91d1\u575a\u6559\u6388\u548c\u54c8\u4f5b\u533b\u5b66\u9662\u7684\u9b4f\u6587\u6bc5\u6559\u6388\uff0c\u957f\u671f\u81f4\u529b\u4e8e\u5f00\u53d1\u65b0\u578bPROTAC\u6280\u672f\u3002\u672c\u6587\u5f00\u53d1\u4e86\u57fa\u4e8eUSP7\u7684DUBTAC\uff0c\u9996\u6b21\u5c55\u793a\u4e86\u53bb\u6cdb\u7d20\u5316\u9176USP7\u53ef\u7528\u4e8eDUBTAC\u7684\u5f00\u53d1\u3002<\/p>\n<section><img decoding=\"async\" title=\"1\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240429\/1714358291217109.png\" alt=\"1\" \/><\/section>\n<p>\u9776\u5411\u86cb\u767d\u8d28\u964d\u89e3\uff08TPD\uff09\u65b9\u6cd5\u53ef\u4ee5\u7528\u4e8e\u964d\u89e3\u5bfc\u81f4\u4eba\u7c7b\u75be\u75c5\u53d1\u5c55\u7684\u86cb\u767d\u8d28\u3002\u7136\u800c\uff0c\u4eba\u7c7b\u75be\u75c5\u662f\u7531\u5177\u6709\u4fdd\u62a4\u529f\u80fd\u7684\u86cb\u767d\u8d28\uff08\u5982\u80bf\u7624\u6291\u5236\u86cb\u767d\uff09\u7684\u8868\u8fbe\u51cf\u5c11\u6216\u529f\u80fd\u4e27\u5931\u5f15\u8d77\u7684\uff0c\u9700\u8981\u7a33\u5b9a\u86cb\u767d\u800c\u4e0d\u662f\u964d\u89e3\u86cb\u767d\u6765\u5bf9\u6297\u75be\u75c5\u3002\u6700\u8fd1\u901a\u8fc7\u5185\u6e90\u6027\u53bb\u6cdb\u7d20\u5316\u9176\u6765\u5f00\u53d1\u53bb\u6cdb\u7d20\u5316\u9176\u9776\u5411\u5d4c\u5408\u4f53\uff08DUBTAC\uff09\u6765\u53bb\u9664\u6cdb\u7d20\u5316\u5e76\u7a33\u5b9a\u611f\u5174\u8da3\u7684\u86cb\u767d\u8d28\u4f5c\u4e3a\u6cbb\u7597\u624b\u6bb5\u3002\u76ee\u524d\u53ea\u6709OTUB1\u53bb\u6cdb\u7d20\u5316\u9176\u901a\u8fc7\u5171\u4ef7\u914d\u4f53EN523\u7528\u4e8e DUBTAC\u5f00\u53d1\uff0c\u7136\u800c\uff0cOTUB1\u5171\u4ef7\u914d\u4f53\u884d\u751f\u7684DUBTAC\u53ef\u80fd\u4e0e\u5176\u4ed6\u5171\u4ef7\u6291\u5236\u5242\u5b58\u5728\u7c7b\u4f3c\u7684\u95ee\u9898\uff0c\u4f8b\u5982\u5f15\u8d77\u6bd2\u6027\u6216\u8d85\u654f\u53cd\u5e94\u3002\u56e0\u6b64\uff0c\u5229\u7528\u5176\u4ed6\u53bb\u6cdb\u7d20\u5316\u9176\u8fdb\u884cDUBTAC\u7684\u5f00\u53d1\u662f\u80fd\u591f\u63a8\u52a8\u9776\u5411\u86cb\u767d\u8d28\u7a33\u5b9a\u9886\u57df\u7684\u53d1\u5c55\u3002<\/p>\n<section><img decoding=\"async\" title=\"2\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240429\/1714358299631172.webp\" alt=\"2\" \/><\/section>\n<section>\u56fe 1. \u57fa\u4e8eUSP7\u7684DUBTAC\u7528\u4e8e\u7a33\u5b9a\u9776\u86cb\u767d<\/p>\n<\/section>\n<p>\u672c\u6587\u62a5\u9053\u4e86\u7b2c\u4e00\u4e2a\u57fa\u4e8eUSP7\u7684DUBTAC\u7684\u5f00\u53d1\uff0c\u8be5DUBTAC\u5229\u7528USP7\u7684\u975e\u5171\u4ef7\u914d\u4f53\u9009\u62e9\u6027\u5730\u7a33\u5b9a\u9776\u86cb\u767d\uff08\u56fe1A\uff09\u3002\u9996\u5148\uff0c\u4f5c\u8005\u9009\u62e9\u5148\u524d\u62a5\u9053\u7684USP7 ligand #1\uff08\u56fe1B\uff09\uff0c\u53ef\u4ee5\u7ed3\u5408\u5e76\u6291\u5236\u7ec6\u80de\u4e2d\u7684USP7\uff0c\u4ee5\u52df\u96c6USP7\u53bb\u6cdb\u7d20\u5316\u9176\u3002USP7 ligand #1\u548cUSP7i-#4a\u590d\u5408\u7269\u7ed3\u6784\u8868\u660e\uff0cUSP7\u6291\u5236\u5242\u7684\u6c28\u57fa\u4e0d\u5728\u7ed3\u5408\u53e3\u888b\u4e2d\uff08\u56fe1B-C\uff09\uff0c\u53ef\u7528\u4f5clinker\u8fde\u63a5\u3002\u7136\u540e\uff0c\u5c06 USP7 ligand #1 \u4e0eCFTR \u914d\u4f53\uff08Lumacaftor\uff09\u4e0e\u5404\u79cd\u8fde\u63a5\u5242\u8fde\u63a5\u8d77\u6765\uff0c\u4ea7\u751f\u5316\u5408\u72691-13\uff08\u56fe2A\uff09\u3002\u5728\u8fd913\u79cd\u5316\u5408\u7269\u4e2d\uff0c\u53ea\u67097\uff08MS6869\uff09\u548c13\u4e24\u79cd\u5316\u5408\u7269\u53ef\u4ee5\u5728\u7ec6\u80de\u4e2d\u7a33\u5b9a\u0394F508-CFTR\u7a81\u53d8\u86cb\u767d\uff08\u56fe2B\uff09\u3002\u4f7f\u7528MS6869\u7814\u7a76\u57fa\u4e8eUSP7\u7684CFTR DUBTAC\u7684\u673a\u5236\uff0c\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0cMS6869\u589e\u52a0\u4e86CFTR\u86cb\u767d\u4e30\u5ea6\uff0c\u4f46\u6ca1\u6709\u589e\u52a0\u5176mRNA\u6c34\u5e73\u3002\u540c\u65f6\uff0cMS6869\u5f15\u5165\u540e\u51fa\u73b0USP7\u548cCFTR\u86cb\u767d\u4e4b\u95f4\u7684\u65b0\u76f8\u4e92\u4f5c\u7528\uff08\u56fe2C\uff09\uff0c\u968f\u540e\u964d\u4f4e\u4e86CFTR\u7684\u6cdb\u7d20\u5316\u6c34\u5e73\uff08\u56fe2D\uff09\u3002\u6b64\u5916\uff0c\u57fa\u4e8eUSP7\u7684CFTR DUBTAC MS6869\u4ee5\u6d53\u5ea6\u548c\u65f6\u95f4\u4f9d\u8d56\u6027\u65b9\u5f0f\u7a33\u5b9aCFTR\uff08\u56fe2E-F\uff09\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0cCFTR\u86cb\u767d\u6c34\u5e73\u7684\u589e\u52a0\u53ef\u80fd\u662f\u529f\u80fd\u6027\u7684\uff0c\u56e0\u4e3a\u5b83\u4e3b\u8981\u4f4d\u4e8e\u8d28\u819c\u4e0a\uff08\u56fe2G\uff09\u3002\u4e0eUSP7\u914d\u4f53\u6216CFTR \u914d\u4f53\u7684\u5171\u540c\u5904\u7406\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u6d88\u9664\u4e86MS6869\u7a33\u5b9a\u0394F508-CFTR \u7a81\u53d8\u86cb\u767d\u7684\u4f5c\u7528\uff08\u56fe2H\uff09\u3002\u540c\u65f6\uff0c\u5728\u7ec6\u80deCFBE41o-4.7\u0394F508-CFTR\u4e2d\u5220\u9664USP7\u53ef\u4ee5\u963b\u65adMS6869\u7a33\u5b9aCFTR\u7684\u4f5c\u7528\uff08\u56fe2I\uff09\uff0c\u8868\u660e MS6869 \u8bf1\u5bfc\u7684CFTR\u7a33\u5b9a\u53ef\u80fd\u4f9d\u8d56\u4e8eUSP7\u7684\u53bb\u6cdb\u7d20\u5316\u9176\u6d3b\u6027\u3002\u6b64\u5916\uff0cTMT\u6807\u8bb0\u8d28\u8c31\uff08MS\uff09\u6d4b\u5b9a\u53d1\u73b0\uff0cMS6869\u4ee5\u76f8\u5bf9\u7279\u5f02\u6027\u7684\u65b9\u5f0f\u4fc3\u8fdbCFTR\u7684\u7a33\u5b9a\uff08\u56fe2J-K\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0cUSP7\u53ef\u7528\u4e8e\u4f7f\u7528\u975e\u5171\u4ef7USP7 \u914d\u4f53\u7a33\u5b9a\u9776\u86cb\u767d\u3002<\/p>\n<section><img decoding=\"async\" title=\"3\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240429\/1714358310990208.webp\" alt=\"3\" \/><\/section>\n<section>\u56fe2.\u57fa\u4e8eUSP7\u7684CFTR DUBTAC\u53ef\u7a33\u5b9a\u7ec6\u80de\u4e2d\u7684\u0394F508-CFTR\u7a81\u53d8\u86cb\u767d<\/p>\n<\/section>\n<p>\u4e3a\u4e86\u8fdb\u4e00\u6b65\u8bc1\u660e\u57fa\u4e8eUSP7\u7684DUBTAC\u53ef\u4ee5\u7a33\u5b9a\u9776\u86cb\u767d\uff0c\u8bbe\u8ba1\u5e76\u5408\u6210\u4e86\u4e00\u7cfb\u5217\u57fa\u4e8eUSP7 \u7684AMPK DUBTACs\uff08\u5316\u5408\u726914-25\uff09\uff0c\u901a\u8fc7\u5c06USP7 ligand #1\u4e0eAMPK\u6fc0\u52a8\u5242991\u8fde\u63a5\u8d77\u6765(\u56fe3A\uff09\u3002AMPK\u6fc0\u52a8\u5242991\u53ef\u4ee5\u7ed3\u5408\u5728AMPK ADaM\u4f4d\u70b9\uff0c\u5e76\u9009\u62e9\u6027\u6fc0\u6d3bAMPK\u03b21\u4e9a\u57fa\u800c\u4e0d\u662fAMPK\u03b22\u4e9a\u57fa\u3002\u6d4b\u91cf\u4e86DUBTACs \u5904\u7406\u540eAMPK\u6fc0\u9176\u7684\u03b1\u548c\u03b2\u4e9a\u57fa\u6c34\u5e73\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u4e09\u79cd\u5316\u5408\u726922\u300123\u548c24\u53ef\u4ee5\u7a33\u5b9aAMPK\u03b21\uff0c\u4f46\u4e0d\u80fd\u7a33\u5b9a<span class=\"wx_search_keyword_wrap\">HeLa<\/span>\u00a0\u7ec6\u80de\u4e2d\u7684AMPK\u03b22\u4e9a\u578b\u548c AMPK\u03b1\u4e9a\u57fa\uff08\u56fe3B\uff09\u3002\u5bf9\u8fd9\u4e9b\u5148\u5bfc\u5316\u5408\u7269\u7684\u8fdb\u4e00\u6b65\u8bc4\u4f30\u8868\u660e\uff0cMS8118\uff08\u5316\u5408\u726924\uff09\u5728\u7a33\u5b9aAMPK\u03b21\u65b9\u9762\u6700\u6709\u6548\uff0c\u5e76\u4f34\u968f\u7740AMPK\u4e0b\u6e38\u5e95\u7269\u4e59\u9170\u8f85\u9176A\u7fa7\u5316\u9176\uff08ACC\uff09\u78f7\u9178\u5316\u5347\u9ad8\uff08\u56fe3C\uff09\uff0c\u800cAMPK\u03b21\u7684mRNA\u6c34\u5e73\u4e0d\u53d7\u5f71\u54cd\uff0c\u8868\u660eAMPK DUBTAC\u5728\u7ffb\u8bd1\u540e\u6c34\u5e73\u8d77\u4f5c\u7528\u3002\u63a5\u4e0b\u6765\uff0c\u9009\u62e9MS8118\u4ee3\u8868 AMPK\u03b21\u8fdb\u884c\u8fdb\u4e00\u6b65\u7684\u529f\u80fd\u6d4b\u5b9a\u3002\u5f15\u5165MS8118\u540eUSP7\u548cAMPK\u03b21 \u4e4b\u95f4\u51fa\u73b0\u65b0\u7684\u76f8\u4e92\u4f5c\u7528\uff08\u56fe3D\uff09\uff0c\u8fd9\u5bfc\u81f4\u7ec6\u80de\u5185AMPK\u03b21 \u6cdb\u7d20\u5316\u6c34\u5e73\u964d\u4f4e\uff08\u56fe3E\uff09\u3002\u6b64\u5916\uff0cMS8118 \u5bf9 AMPK\u03b21 \u7a33\u5b9a\u7684\u4f5c\u7528\u53ef\u80fd\u4f1a\u88abUSP7\u914d\u4f53\u548cAMPK\u914d\u4f53\uff08\u56fe3F\uff09\u6216\u901a\u8fc7\u6d88\u8017\u5185\u6e90\u6027USP7\u963b\u65ad\uff08\u56fe3G\uff09\uff0c\u8868\u660eMS8118\u5bf9AMPK\u03b21\u86cb\u767d\u7a33\u5b9a\u6027\u7684\u5f71\u54cd\u53ef\u80fd\u662f\u901a\u8fc7\u52df\u96c6USP7\u53bb\u6cdb\u7d20\u5316AMPK\u03b21\u3002\u4e3a\u4e86\u8fdb\u4e00\u6b65\u786e\u5b9aDUBTAC\u8bf1\u5bfc\u7684AMPK\u03b21\u7a33\u5b9a\u662f\u5426\u4f1a\u5f71\u54cd\u7ec6\u80de\u529f\u80fd\uff0c\u8fdb\u884c\u4e86Oil Red O\u67d3\u8272\u6d4b\u5b9a\u4f5c\u4e3a\u4e0b\u6e38\u8bfb\u6570\u3002\u4e0eAMPK\u03b21\u7a33\u5b9a\u7ed3\u679c\u4e00\u81f4\uff0cMS8118\u4ee5\u53ca\u5316\u5408\u726923\u5728\u8f83\u5c0f\u7a0b\u5ea6\u4e0a\u635f\u5bb3\u4e86HeLa\u7ec6\u80de\u4e2d\u7684\u8102\u8d28\u79ef\u7d2f\uff08\u56fe3H\uff09\u3002\u9274\u4e8eAMPK\u5728\u764c\u75c7\u4e2d\u4e5f\u53d1\u6325\u7ec6\u80de\u73af\u5883\u4f9d\u8d56\u6027\u80bf\u7624\u6291\u5236\u4f5c\u7528\uff0c\u8fdb\u4e00\u6b65\u786e\u5b9a\u4e86\u8fd9\u4e9bAMPK DUBTAC\u662f\u5426\u5f71\u54cd\u80bf\u7624\u7ec6\u80de\u751f\u957f\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u5728\u96c6\u843d\u5f62\u6210\u8bd5\u9a8c\u4e2dMS8118\u300122 \u6216 23 \u51cf\u5c0f\u4e86\u96c6\u843d\u5927\u5c0f\uff08\u56fe 3I\uff09\u3002<\/p>\n<section><img decoding=\"async\" title=\"4\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240429\/1714358319194407.webp\" alt=\"4\" \/><\/section>\n<section>\u56fe3. USP7 ligand #1\u884d\u751f\u7684AMPK DUBTAC\u53ef\u7a33\u5b9a AMPK\u03b21\u5e76\u6fc0\u6d3b\u7ec6\u80de\u4e2d\u7684\u4e0b\u6e38AMPK\u4fe1\u53f7\u8f6c\u5bfc<\/p>\n<\/section>\n<p>\u63a5\u4e0b\u6765\uff0c\u4f7f\u7528\u53e6\u4e00\u79cdUSP7\u975e\u5171\u4ef7\u6291\u5236\u5242GNE6776\uff08\u56fe1C\uff09\uff0c\u5b83\u4e0eAMPK\u4e9a\u57fa\u5177\u6709\u4e0d\u540c\u7684\u7ed3\u5408\u6a21\u5f0f\uff0c\u53ef\u4ee5\u6291\u5236\u7ec6\u80de\u4e2d\u7684USP7\uff0c\u4f5c\u4e3aUSP7\u914d\u4f53\u4ea7\u751f\u4e00\u7cfb\u5217\u65b0\u7684\u57fa\u4e8eUSP7\u7684AMPK DUBTAC\u5316\u5408\u726926-36\uff08\u56fe4A\uff09\u3002\u4e0eUSP7 ligand #1\u884d\u751f\u7684AMPK DUBTAC\u76f8\u6bd4\uff0cGNE6776\u884d\u751f\u7684AMPK DUBTAC\u5bf9AMPK\u03b21\u7684\u7a33\u5b9a\u6027\u5f71\u54cd\u6700\u5c0f\uff08\u56fe 4B\uff09\u3002\u76f8\u53cd\uff0c55\uff08MS8655\uff09\u300156\uff08MS8656\uff09 \u548c 57\uff08MS8657\uff09 \u4e09\u79cd\u5316\u5408\u7269\u4f18\u5148\u589e\u52a0\u4e86HeLa\u7ec6\u80de\u4e2dAMPK\u03b22\u7684\u86cb\u767d\u7a33\u5b9a\u6027\uff08\u56fe4B\uff09\u3002\u8fdb\u4e00\u6b65\u7684\u5206\u6790\u8868\u660e\uff0cMS8655\u3001MS8656 \u548c MS8657 \u4e3b\u8981\u4ee5\u6d53\u5ea6\u4f9d\u8d56\u6027\u65b9\u5f0f\u7a33\u5b9a AMPK\u03b22\uff0c\u800cAMPK\u03b21\u4ec5\u53d7\u5230\u4e2d\u5ea6\u5f71\u54cd\uff08\u56fe4C\uff09\u3002\u6b64\u5916\uff0cAMPK\u03b22\u7684mRNA \u6c34\u5e73\u4e0d\u53d7\u4e09\u79cdAMPK-DUBTAC\uff08MS8655\u3001MS8656 \u548c MS8657\uff09\u7684\u5f71\u54cd\uff0c\u8868\u660e\u8fd9\u4e9b\u5316\u5408\u7269\u5bf9AMPK\u03b22\u7684\u7ffb\u8bd1\u540e\u8c03\u63a7\u3002\u63a5\u4e0b\u6765\uff0c\u4f7f\u7528AMPK\u03b22\u7684\u4e00\u4e2a\u4ee3\u8868\u6027DUBTAC\uff08MS8657\uff09 \u88ab\u7528\u4e8e\u8fdb\u4e00\u6b65\u7684\u529f\u80fd\u6d4b\u5b9a\u3002\u4e0eMS8118 \u7c7b\u4f3c\uff0cMS8657\u5f15\u5165\u4e86USP7\u548cAMPK\u03b22\u4e4b\u95f4\u7684\u65b0\u76f8\u4e92\u4f5c\u7528\uff08\u56fe4D\uff09\uff0c\u968f\u540e\u964d\u4f4e\u4e86AMPK\u03b22\u7684\u6cdb\u7d20\u5316\u6c34\u5e73\uff08\u56fe4E\uff09\u3002\u6b64\u5916\uff0cMS8657\u5bf9AMPK\u03b22\u7a33\u5b9a\u4f5c\u7528\u88ab USP7 \u548c AMPK \u7684\u914d\u4f53\uff08\u56fe4F\uff09\u6216\u901a\u8fc7\u5185\u6e90\u6027USP7\u7684\u5220\u9664\u963b\u65ad\uff08\u56fe4G\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u8bc1\u660eAMPK-DUBTAC\u4ee5USP7\u4f9d\u8d56\u6027\u65b9\u5f0f\u7a33\u5b9a\u86cb\u767d\u5e95\u7269\u3002\u4e3a\u4e86\u4e0eAMPK-DUBTAC\u7a33\u5b9aAMPK\u03b22\u4e2d\u7684\u4f5c\u7528\u4fdd\u6301\u4e00\u81f4\uff0c\u8fd9\u4e9b DUBTAC \u8fd8\u5bfc\u81f4Oil Red O\u67d3\u8272\u8bc4\u5206\u964d\u4f4e\uff0c\u8868\u660eHeLa\u7ec6\u80de\u4e2d\u7684\u8102\u8d28\u79ef\u7d2f\u88ab\u7834\u574f\uff08\u56fe4H\uff09\u3002\u6b64\u5916\uff0cMS8655\u3001MS8656 \u548c MS8657 \u5904\u7406\u51cf\u5c11\u4e86HeLa\u7ec6\u80de\u7684\u96c6\u843d\u6570\u91cf\u548c\u96c6\u843d\u5927\u5c0f\uff08\u56fe4I\uff09\u3002\u603b\u4e4b\uff0c\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0cUSP7\u53ef\u4ee5\u7528\u4e8e\u751f\u6210AMPK DUBTACs\uff0c\u4ece\u800c\u6709\u6548\u7a33\u5b9aAMPK\u03b22\uff0c\u6fc0\u6d3bAMPK\u4fe1\u53f7\u4f20\u5bfc\uff0c\u51cf\u5c11\u80bf\u7624\u7ec6\u80de\u751f\u957f\u3002<\/p>\n<section><img decoding=\"async\" title=\"6\" src=\"https:\/\/17332.seohost.cn\/storage\/17332\/article\/20240429\/1714358332993769.webp\" alt=\"6\" \/>\u200b<\/section>\n<p>\u56fe4. USP7 ligand #2\uff0cGNE6776\u884d\u751f\u7684USP7-AMPK DUBTAC\u7a33\u5b9a\u7ec6\u80de\u4e2d\u7684AMPK\u03b22<\/p>\n<p>\u672c\u6587\u57fa\u4e8eUSP7\u7684\u975e\u5171\u4ef7\u914d\u4f53\uff0c\u5f00\u53d1\u4e86\u57fa\u4e8eUSP7\u7684DUBTAC\uff0c\u5176\u7a33\u5b9a\u0394F508-CFTR\u7a81\u53d8\u86cb\u767d\u4e0e\u4e4b\u524d\u62a5\u9053\u7684\u57fa\u4e8eOTUB1\u7684DUBTAC\u5177\u6709\u76f8\u4f3c\u7684\u6548\u679c\u3002\u91cd\u8981\u7684\u662f\uff0c\u4f7f\u7528\u4e24\u79cd\u4e0d\u540c\u7684USP7\u975e\u5171\u4ef7\u914d\u4f53\uff0c\u5f00\u53d1\u4e86\u7b2c\u4e00\u4e2aAMPK DUBTAC\uff0c\u8fd9\u4e9bAMPK\u4f3c\u4e4e\u53ef\u4ee5\u9009\u62e9\u6027\u5730\u7a33\u5b9aAMPK\u03b2\u7684\u4e0d\u540c\u4e9a\u578b\uff0c\u5bfc\u81f4AMPK\u4fe1\u53f7\u8f6c\u5bfc\u5347\u9ad8\u3002\u8fd9\u4e9b\u7ed3\u679c\u5f3a\u8c03\uff0c\u9664\u4e86OTUB1\u4e4b\u5916\uff0cUSP7\u8fd8\u53ef\u4ee5\u7528\u4e8e\u5f00\u53d1DUBTAC\uff0c\u4ece\u800c\u6269\u5c55\u4e86\u9776\u5411\u86cb\u767d\u8d28\u7a33\u5b9a\u548c\u5f00\u53d1\u65b0\u578b AMPK DUBTAC \u4f5c\u4e3a\u6f5c\u5728\u7597\u6cd5\u7684\u5de5\u5177\u7bb1\u3002<\/p>\n<section>\u6587\u7ae0\u4f5c\u8005\uff1aYJ<\/p>\n<p>\u539f\u6587\u5f15\u7528\uff1a<\/p>\n<p>https:\/\/doi.org\/10.1021\/jacs.4c02373<\/p>\n<p>\u8d23\u4efb\u7f16\u8f91\uff1aLD<\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u5206\u4eab\u4e00\u7bc7\u53d1\u8868\u5728JACS\u6742\u5fd7\u4e0a\u7684\u6587\u7ae0\uff0c\u9898\u76ee\u4e3a\u201cUSP7-Based Deubiquitinase-Targeti [&hellip;]<\/p>\n","protected":false},"author":326,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-51392","post","type-post","status-publish","format-standard","hentry","category-tuijianyuedu"],"_links":{"self":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51392","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/users\/326"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/comments?post=51392"}],"version-history":[{"count":1,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51392\/revisions"}],"predecessor-version":[{"id":51393,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/51392\/revisions\/51393"}],"wp:attachment":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/media?parent=51392"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/categories?post=51392"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/tags?post=51392"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}