{"id":51325,"date":"2024-04-27T08:48:19","date_gmt":"2024-04-27T00:48:19","guid":{"rendered":"https:\/\/www.chemhui.com\/?p=51325"},"modified":"2024-04-18T10:51:08","modified_gmt":"2024-04-18T02:51:08","slug":"angew%e7%8e%af%e4%b8%99%e7%83%af%e4%bd%9c%e4%b8%ba%e5%ae%9e%e7%8e%b0dna%e5%8f%8c%e9%87%8d%e7%94%9f%e7%89%a9%e6%ad%a3%e4%ba%a4%e4%bb%a3%e8%b0%a2%e6%a0%87%e8%ae%b0%e7%9a%84%e5%8c%96%e5%ad%a6%e6%8e%a2","status":"publish","type":"post","link":"http:\/\/www.chemhui.com\/angew%e7%8e%af%e4%b8%99%e7%83%af%e4%bd%9c%e4%b8%ba%e5%ae%9e%e7%8e%b0dna%e5%8f%8c%e9%87%8d%e7%94%9f%e7%89%a9%e6%ad%a3%e4%ba%a4%e4%bb%a3%e8%b0%a2%e6%a0%87%e8%ae%b0%e7%9a%84%e5%8c%96%e5%ad%a6%e6%8e%a2\/","title":{"rendered":"Angew|\u73af\u4e19\u70ef\u4f5c\u4e3a\u5b9e\u73b0DNA\u53cc\u91cd\u751f\u7269\u6b63\u4ea4\u4ee3\u8c22\u6807\u8bb0\u7684\u5316\u5b66\u63a2\u9488"},"content":{"rendered":"<p>\u63a8\u8350\u4e00\u7bc7\u53d1\u8868\u5728Angewandte Chemie International Edition\u4e0a\u7684\u6587\u7ae0\uff0c\u6587\u7ae0\u6807\u9898\u662f\u201cCyclopropenes as Chemical Reporters for Dual Bioorthogonal and Orthogonal Metabolic Labeling of DNA\u201d\u3002\u5176\u901a\u8baf\u4f5c\u8005\u662f\u5361\u5c14\u65af\u9c81\u5384\u7406\u5de5\u5b66\u9662\u7684 Hans-Achim Wagenknecht \u6559\u6388\uff0c\u81f4\u529b\u4e8e\u6838\u9178\u5316\u5b66\u3001\u5316\u5b66\u5149\u50ac\u5316\u3001\u8367\u5149DNA\u548cRNA\u3001DNA\u7ed3\u6784\u5b66\u3002<\/p>\n<section><img decoding=\"async\" title=\"1\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20240418\/1713408571529348.png\" alt=\"1\" 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class=\"wx_search_keyword_wrap\">\u751f\u7269\u6b63\u4ea4\u53cd\u5e94<\/span>\u6269\u5c55\u5230\u591a\u4e2a\u76ee\u6807\u3002\u7136\u800c\uff0c\u5728\u540c\u4e00\u73af\u5883\u4e2d\u540c\u65f6\u5e94\u7528\u591a\u79cd\u751f\u7269\u6b63\u4ea4\u53cd\u5e94\u4ecd\u7136\u5177\u6709\u6311\u6218\u6027\uff0c\u56e0\u4e3a\u53ef\u80fd\u4f1a\u53d1\u751f\u4ea4\u53c9\u53cd\u5e94\u3002<span class=\"wx_text_underline\">\u76ee\u524d\uff0c\u4e3a\u4e86\u5b9e\u73b0\u53cc\u91cd\u6216\u4e09\u91cd\u751f\u7269\u6b63\u4ea4\u6807\u8bb0\uff0c\u4e3b\u8981\u91c7\u7528\u4e24\u79cd\u65b9\u6cd5\uff1a\u4e00\u662f\u57fa\u4e8e\u4e0d\u540c\u7684\u53cd\u5e94\u673a\u7406\uff0c\u4e8c\u662f\u8c03\u63a7\u5316\u5b66\u62a5\u544a\u57fa\u56e2\u7684\u53cd\u5e94\u6027\u3002<\/span><\/p>\n<p>\u5728\u5148\u524d\u7684\u7814\u7a76\u4e2d\uff0c\u4f5c\u8005\u5df2\u7ecf\u6210\u529f\u5730\u5229\u7528<span class=\"wx_search_keyword_wrap\">1-\u7532\u57fa\u73af\u4e19\u70ef<\/span>\u4fee\u9970\u7684DNA\u901a\u8fc7IEDDA\u53cd\u5e94\u5b9e\u73b0\u4e86\u6807\u8bb0\u3002\u4e8e\u662f\uff0c\u4f5c\u8005\u5e0c\u671b\u5728\u4e4b\u524d\u7814\u7a76\u7684\u57fa\u7840\u4e0a\u5b9e\u73b0DNA\u7684\u53cc\u91cd\u751f\u7269\u6b63\u4ea4\u6807\u8bb0\u3002\u4e4b\u524d\u7684\u7814\u7a76\u8868\u660e\uff0c\u7532\u57fa\u73af\u4e19\u70ef\u7684\u53cd\u5e94\u6027\u4f1a\u968f\u7740\u7532\u57fa\u53d6\u4ee3\u57fa\u7684\u4f4d\u7f6e\u800c\u6539\u53d8\uff0c\u4f8b\u59821-\u7532\u57fa\u73af\u4e19\u70ef\u662f\u5e94\u7528\u4e8eIEDDA\u53cd\u5e94\u7684\u7406\u60f3\u5019\u9009\u7269\uff0c\u800c3-\u7532\u57fa\u73af\u4e19\u70ef\u7684\u7532\u57fa\u57fa\u56e2\u4f1a\u5728IEDDA\u53cd\u5e94\u7684\u8fc7\u6e21\u6001\u4e2d\u9020\u6210\u7a7a\u95f4\u4f4d\u963b\uff0c\u4f46\u5374\u53ef\u4ee5\u4e0e\u56db\u5511\u539f\u4f4d\u4ea7\u751f\u7684\u8148\u4e9a\u80fa\u53d1\u751f\u5149\u70b9\u51fb\u53cd\u5e94\uff08\u56fe1A\uff09\u3002\u57fa\u4e8e\u8fd9\u4e00\u7406\u8bba\uff0c\u4f5c\u8005\u8bbe\u8ba1\u4e86\u4e00\u7cfb\u5217\u542b\u67091-\u7532\u57fa\u73af\u4e19\u70ef\/3-\u7532\u57fa\u73af\u4e19\u70ef\u7684\u63a2\u9488\uff08\u56fe1B\uff09\uff0c\u5e0c\u671b\u80fd\u591f\u901a\u8fc7IEDDA\u53cd\u5e94\u548c\u5149\u70b9\u51fb\u53cd\u5e94\u5b9e\u73b0DNA\u7684\u53cc\u91cd\u751f\u7269\u6b63\u4ea4\u6807\u8bb0\uff08\u56fe1C\uff09\u3002<\/p>\n<\/section>\n<section><img decoding=\"async\" title=\"2\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20240418\/1713408581799917.webp\" alt=\"2\" \/><\/section>\n<section>\u56fe1 A\uff1a\u56db\u5511\u7c7b\u5316\u5408\u7269\u7684\u5149\u70b9\u51fb\u53cd\u5e94<\/p>\n<p>B\uff1a1-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684\u8131\u6c27\u6838\u7cd6\u6838\u82f7\uff081-5\uff09\u7528\u4e8eIEDDA\u53cd\u5e94\uff0c3-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684\u8131\u6c27\u6838\u7cd6\u6838\u82f7\uff086-7\uff09\u7528\u4e8e\u5149\u70b9\u51fb\u53cd\u5e94<\/p>\n<p>C\uff1a\u7ed3\u5408IEDDA\u53cd\u5e94\u548c\u5149\u70b9\u51fb\u53cd\u5e94\u5bf9DNA\u8fdb\u884c\u53cc\u91cd\u751f\u7269\u6b63\u4ea4\u6807\u8bb0<\/p>\n<\/section>\n<section>\u5728\u8fd9\u9879\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u7684\u8bbe\u60f3\u662f\u542b\u67091-\u7532\u57fa\u73af\u4e19\u70ef\u62163-\u7532\u57fa\u73af\u4e19\u70ef\u7684\u63a2\u9488\u5728IEDDA\u53cd\u5e94\u6216\u5149\u70b9\u51fb\u53cd\u5e94\u8fbe\u5230\u6700\u5feb\u53cd\u5e94\u901f\u7387\u7684\u524d\u63d0\u4e0b\u5b9e\u73b0\u4e24\u8005\u7684\u6b63\u4ea4\u6807\u8bb0\u3002\u57fa\u4e8e\u6b64\u60f3\u6cd5\uff0c\u4f5c\u8005\u9996\u5148\u5728\u4f53\u5916\u5229\u7528\u4e8c\u5421\u5576\u53d6\u4ee3\u7684\u56db\u55ea8\/\u4e8c\u82b3\u57fa\u56db\u55119\u5bf9\u4e0d\u540c\u63a2\u9488\u7684\u53cd\u5e94\u901f\u7387\u8fdb\u884c\u6d4b\u5b9a\uff08\u56fe2A\uff09\uff0c\u7ed3\u679c\u8868\u660e\u63a2\u94881\u548c6\u5206\u522b\u5728IEDDA\u53cd\u5e94\u548c\u5149\u70b9\u51fb\u53cd\u5e94\u4e2d\u8fbe\u5230\u6700\u5feb\u53cd\u5e94\u901f\u7387\uff0c\u968f\u540e\u4f5c\u8005\u5728\u4f53\u5916\u91c7\u7528\u8fd9\u4e24\u79cd\u63a2\u9488\u6765\u8bc4\u4ef7\u4e24\u8005\u53cd\u5e94\u7684\u6b63\u4ea4\u6027\u3002\u4f5c\u8005\u5c06\u63a2\u94881\u548c6\u7b49\u6469\u5c14\u6df7\u5408\u5728PBS:<span class=\"wx_search_keyword_wrap\">MeCN<\/span>\uff081:1\uff09\u4e2d\uff0c\u968f\u540e\u5206\u522b\u4e0e\u4e8c\u5421\u5576\u53d6\u4ee3\u7684\u56db\u55ea8\u3001\u4e8c\u82b3\u57fa\u56db\u55119\u8fdb\u884c\u53cd\u5e94\uff0c\u5e76\u901a\u8fc7HPLC\u4e0eLC-MS\u5bf9\u53cd\u5e94\u7684\u9009\u62e9\u6027\u8fdb\u884c\u8bc4\u4f30\uff0c\u7ed3\u679c\u8868\u660e\u63a2\u94881\u548c6\u4e24\u8005\u7684\u53cd\u5e94\u76f8\u4e92\u6b63\u4ea4\uff08\u56fe2B\uff09\u3002<\/p>\n<\/section>\n<section><img decoding=\"async\" title=\"3\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20240418\/1713408591918457.webp\" alt=\"3\" \/><\/section>\n<section>\u56fe2 A\uff1a\u63a2\u94881-5\u4e0e\u56db\u55ea8\u3001\u63a2\u94886-7\u4e0e\u56db\u55119\u53cd\u5e94\u7684\u4e8c\u7ea7\u901f\u7387\u5e38\u6570<\/p>\n<p>B\uff1a\u7528HPLC\u6765\u5206\u6790\u63a2\u94881\u548c6\u7684\u6b63\u4ea4\u53cd\u5e94\u6027<\/p>\n<\/section>\n<section>\u63a5\u7740\u4f5c\u8005\u5728\u4f53\u5916DNA\u6c34\u5e73\u4e0a\u8bbe\u8ba1\u4e86\u5f15\u7269\u5ef6\u4f38\u5b9e\u9a8c\u4ee5\u53ca\u540e\u7eed\u7684\u8367\u5149\u6807\u8bb0\u5b9e\u9a8c\uff0c\u9a8c\u8bc1\u6240\u7b5b\u9009\u7684\u4e24\u79cd\u63a2\u9488\u5728DNA\u590d\u5236\u65f6\u662f\u5426\u53ef\u4ee5\u88abDNA\u805a\u5408\u9176\u8bc6\u522b\u4ee5\u53ca\u662f\u5426\u53ef\u4ee5\u5728DNA\u6c34\u5e73\u4e0a\u5b9e\u73b0IEDDA\u53cd\u5e94\u6216\u8005\u5149\u70b9\u51fb\u53cd\u5e94\u3002\u4f5c\u8005\u5728\u5f15\u7269\u5ef6\u4f38\u5b9e\u9a8c\u4e2d\u9009\u7528\u4e86Cy5\/ ATTO 390\u6807\u8bb0\u7684\u5f15\u7269P1\/P2\u3001\u6a21\u677fT1\u3001\u4e09\u79cd\u5e02\u552e\u7684DNA\u805a\u5408\u9176\uff0c\u5f53\u5f15\u7269P1\u4e0e\u6a21\u677fT1\u7ed3\u5408\u540e\uff0c\u5728\u542b\u6709\u4e0d\u540c\u5e95\u7269\u7684\u7ec4\uff08\u9634\u6027\u7ec4\uff1a\u542b\u6709\u672a\u4fee\u9970\u7684dATP\u3001dCTP\u3001dGTP\uff0c\u9633\u6027\u7ec4\uff1a\u542b\u6709\u672a\u4fee\u9970\u7684dATP\u3001dCTP\u3001dGTP\u3001<span class=\"wx_search_keyword_wrap\">dTTP<\/span>\uff0c\u5b9e\u9a8c\u7ec4\uff1a\u542b\u6709\u672a\u4fee\u9970\u7684dATP\u3001dCTP\u3001dGTP\u548c1-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684dTTP\/3-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684dTTP\uff09\u4e2dDNA\u805a\u5408\u9176\u5bf9\u5f15\u7269P1\u5ef6\u4f385min\uff0c\u968f\u540e\u7528PAGE\u8fdb\u884c\u5206\u6790\uff0c\u7ed3\u679c\u8868\u660e\u5e26\u67091-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684dTTP\u6216\u80053-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684dTTP\u53ef\u4ee5\u88abDNA\u805a\u5408\u9176\u8bc6\u522b\uff08\u56fe3A\u3001B\uff09\u3002\u4f5c\u8005\u968f\u540e\u7528Deep Vent (exo-)\u805a\u5408\u9176\u5728\u5e95\u726910\/12\u7684\u5b58\u5728\u4e0b\u5bf9\u5f15\u7269P1\u5ef6\u4f3830 min\uff0c\u63a5\u7740\u5c061-\u7532\u57fa\u73af\u4e19\u70ef\u6216\u80053-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684\u5be1\u6838\u82f7\u9178\u8fdb\u884c\u8131\u76d0\u3001\u51bb\u5e72\u3001\u6eb6\u89e3\uff0c\u5e76\u5206\u522b\u7528\u67d3\u659911\u548c13\u8fdb\u884c\u6807\u8bb0\uff0c\u901a\u8fc7PAGE\u5206\u6790\u3002\u7ed3\u679c\u8868\u660e1-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684\u5be1\u6838\u82f7\u9178\u53ef\u4ee5\u6210\u529f\u8fdb\u884cIEDDA\u53cd\u5e94\uff08\u56fe3C\uff09\u800c3-\u7532\u57fa\u73af\u4e19\u70ef\u4fee\u9970\u7684\u5be1\u6838\u82f7\u9178\u672a\u6210\u529f\u8fdb\u884c\u5149\u70b9\u51fb\u53cd\u5e94\uff08\u56fe3D\uff09\u3002\u5bf9\u4e8e\u672a\u6210\u529f\u8fdb\u884c\u7684\u5149\u70b9\u51fb\u53cd\u5e94\u53ef\u80fd\u662f\u7531\u4e8e\u9009\u62e9\u7684\u6eb6\u5242\u65e0\u6cd5\u540c\u65f6\u6ee1\u8db3\u53cd\u5e94\u6761\u4ef6\u548c\u5be1\u6838\u82f7\u9178\u6837\u54c1\u7684\u6eb6\u89e3\u6027\uff0c\u8fd9\u4e5f\u5e2e\u52a9\u4f5c\u8005\u8c03\u6574\u540e\u7eed\u5728\u7ec6\u80de\u6c34\u5e73\u4e0a\u5b9e\u73b0\u5149\u70b9\u51fb\u53cd\u5e94\u7684\u65b9\u6848\u3002<\/p>\n<\/section>\n<section><img decoding=\"async\" title=\"4\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20240418\/1713408600387172.webp\" alt=\"4\" \/><\/section>\n<section>\u56fe3 A\u3001B\uff1a\u5f15\u7269P1\u4e0e\u6a21\u677fT1\u7ed3\u5408\u540e\uff0c\u9a8c\u8bc1\u4e09\u79cd\u5e02\u552eDNA\u805a\u5408\u9176\u662f\u5426\u53ef\u4ee5\u8bc6\u522b\u5e26\u6709\u4e24\u79cd\u4fee\u9970\u7684\u8131\u6c27\u6838\u7cd6\u6838\u82f7\u4e09\u78f7\u9178<\/p>\n<p>C\uff1ab\uff09\u3001c\uff09\u5206\u522b\u5bf9\u5e94\u4e8e\u4fee\u9970\u6709Cy5\u7684\u5f15\u7269P1\u3001\u6210\u529f\u8fdb\u884cIEDDA\u53cd\u5e94\u7684\u5be1\u6838\u82f7\u9178\u7684\u51dd\u80f6\u5185\u8367\u5149\u6210\u50cf\uff0cd\uff09\u4e24\u8005\u51dd\u80f6\u5185\u8367\u5149\u6210\u50cf\u7684Merge<\/p>\n<p>D\uff1a\u5bf9\u5e94\u4e8e\u4fee\u9970\u6709ATTO 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