{"id":49537,"date":"2023-12-17T08:42:30","date_gmt":"2023-12-17T00:42:30","guid":{"rendered":"https:\/\/www.chemhui.com\/?p=49537"},"modified":"2023-11-30T09:42:58","modified_gmt":"2023-11-30T01:42:58","slug":"acs-cent-sci-%e5%9f%ba%e4%ba%8e%e6%b7%b1%e5%ba%a6%e5%ad%a6%e4%b9%a0%e7%9a%84%e6%96%87%e5%ba%93%e8%ae%be%e8%ae%a1%e7%94%a8%e4%ba%8e%e4%bb%8e%e5%a4%b4%e5%8f%91%e7%8e%b0%e7%94%9f%e7%89%a9%e6%b4%bb","status":"publish","type":"post","link":"http:\/\/www.chemhui.com\/acs-cent-sci-%e5%9f%ba%e4%ba%8e%e6%b7%b1%e5%ba%a6%e5%ad%a6%e4%b9%a0%e7%9a%84%e6%96%87%e5%ba%93%e8%ae%be%e8%ae%a1%e7%94%a8%e4%ba%8e%e4%bb%8e%e5%a4%b4%e5%8f%91%e7%8e%b0%e7%94%9f%e7%89%a9%e6%b4%bb\/","title":{"rendered":"ACS Cent. Sci. | \u57fa\u4e8e\u6df1\u5ea6\u5b66\u4e60\u7684\u6587\u5e93\u8bbe\u8ba1\u7528\u4e8e\u4ece\u5934\u53d1\u73b0\u751f\u7269\u6d3b\u6027\u786b\u80bd"},"content":{"rendered":"<p>\u63a8\u8350\u4e00\u7bc7\u53d1\u8868\u5728ACS Cent. Sci.\u4e0a\u7684\u8bba\u6587\uff0c\u9898\u76ee\u4e3a\u201cDeep Learning-Driven Library Design for the De Novo Discovery of Bioactive Thiopeptides\u201d\uff0c\u672c\u6587\u7684\u901a\u8baf\u4f5c\u8005\u662f\u6765\u81ea\u4e1c\u4eac\u5927\u5b66\u5316\u5b66\u7cfb\u540c\u8bfe\u9898\u7ec4\u7684Yuki Goto\u6559\u6388\u3001Hiroaki Suga\u6559\u6388\u4ee5\u53caAlexander A.Vinogradov\uff0cYuki Goto\u6559\u6388\u548cHiroaki Suga\u6559\u6388\u4e3b\u8981\u7814\u7a76\u65b9\u5411\u4e3a\u9057\u4f20\u5bc6\u7801\u5b50\u91cd\u7f16\u7a0b\u4ee5\u53ca\u975e\u6807\u80bd\u7684\u6838\u7cd6\u4f53\u5408\u6210\u3002<\/p>\n<p>&nbsp;<\/p>\n<section><img decoding=\"async\" title=\"1\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20231124\/1700793902945788.png\" alt=\"1\" \/><\/section>\n<p>&nbsp;<\/p>\n<p>\u6838\u7cd6\u4f53\u5408\u6210\u9176\u548c\u7ffb\u8bd1\u540e\u4fee\u9970\u80bd\uff08Ribosomally synthesized and post-translationally modified peptides\uff0cRiPP\uff09\u662f\u4e00\u7c7b\u5177\u6709\u7ed3\u6784\u548c\u529f\u80fd\u591a\u6837\u6027\u7684\u5929\u7136\u4ea7\u7269\u3002<strong>RiPP\u751f\u7269\u5408\u6210\u9176\u5177\u6709\u5e7f\u6cdb\u7684\u5e95\u7269\u8010\u53d7\u6027\uff0c\u8fd9\u4e3aRiPP\u5de5\u7a0b\u63d0\u4f9b\u4e86\u8bb8\u591a\u6709\u6548\u7684\u7b56\u7565\u3002\u7136\u800c\uff0c\u5c3d\u7ba1RiPP\u751f\u7269\u5408\u6210\u9176\u5177\u6709\u5bbd\u677e\u7684\u5e95\u7269\u7279\u5f02\u6027\uff0c\u5b83\u7684\u5e95\u7269\u504f\u597d\u6027\u662f\u96be\u4ee5\u786e\u5b9a\u7684\u3002\u56e0\u6b64\uff0c\u5728\u8bbe\u8ba1RiPP\u524d\u4f53\u7684\u7ec4\u5408\u6587\u5e93\u65f6\uff0c\u9700\u8981\u8003\u8651\u5230\u5316\u5408\u7269\u591a\u6837\u6027\u4ee5\u53ca\u5e95\u7269\u9002\u5e94\u6027\u3002\u7136\u800c\uff0c\u8981\u5728\u4e8c\u8005\u4e4b\u95f4\u53d6\u5f97\u5e73\u8861\u4ecd\u5177\u6709\u6311\u6218\u6027\u3002<\/strong><\/p>\n<p>&nbsp;<\/p>\n<section><img decoding=\"async\" title=\"2\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20231124\/1700793910339799.png\" alt=\"2\" \/><\/section>\n<p>&nbsp;<\/p>\n<p>Lactazole A\u662f\u4e73\u9178\u94fe\u9709\u83cc\uff08Streptomyces lactacystinaeus\uff09\u7684\u4e00\u79cd\u786b\u80bd\uff0c\u6709\u4e94\u79cd\u9176LazBCDEF \u53c2\u4e0e\u5176\u751f\u7269\u5408\u6210\u3002\u5728\u8fd9\u7bc7\u6587\u7ae0\u4e2d\uff0c\u4f5c\u8005\u91c7\u7528\u6df1\u5ea6\u5b66\u4e60\u6a21\u578b\u4ee5\u5b9e\u73b0\u57fa\u4e8eLazA\u7684mRNA\u6587\u5e93\u7684\u8bbe\u8ba1\u3002\u9996\u5148\uff0c\u4f5c\u8005\u6784\u5efa\u968f\u673a\u7684LazA\u6587\u5e93\uff0c\u91c7\u7528\u4f53\u5916\u7ffb\u8bd1\uff08FIT\uff09\u548cmRNA\u5c55\u793a\uff0c\u5c06\u5408\u6210\u7684\u591a\u80bd\u901a\u8fc7puromycin\u4e0emRNA\u76f8\u8fde\uff0c\u5e76\u5c06N\u03b1-\u751f\u7269\u7d20\u5316\u7684Phe\u4f5c\u4e3aN\u672b\u7aef\uff0c\u4f7f\u7528Laz\u9176\u5904\u7406\u4ee5\u53caHA\u7eaf\u5316\u540e\uff0c\u5b8c\u5168\u6210\u719f\u7684\u5e95\u7269\u5c06\u4f1a\u5931\u53bbN\u672b\u7aef\u7684\u751f\u7269\u7d20\u6807\u7b7e\u3002\u7136\u540e\u901a\u8fc7\u94fe\u9709\u7d20\u548c\u7d20\u78c1\u73e0\u8fdb\u884c\u5bcc\u96c6\uff0c\u5176\u4e2d\u7ebf\u578b\u5f62\u5f0f\u7684LazA\u7a81\u53d8\u4f53\u88ab\u78c1\u73e0\u4fdd\u7559\uff0c\u800c\u73af\u5316\u4e3a\u786b\u80bd\u7684LazA\u7a81\u53d8\u4f53\u5219\u4fdd\u7559\u5728\u6eb6\u6db2\u4e2d\u3002\u5176\u4e2d\u5b8c\u5168\u4fee\u9970\u7684\u786b\u80bd\u7684\u672a\u7ed3\u5408\u90e8\u5206\u548c\u7ebf\u578b\u5f62\u5f0f\u7684\u80bd\u7684\u7ed3\u5408\u90e8\u5206\u5728\u6bcf\u4e00\u8f6e\u4e2d\u90fd\u88ab\u56de\u6536\u548c\u6269\u589e\uff0c\u5171\u8fdb\u884c\u516d\u8f6e\uff0c\u4ee5\u83b7\u5f97\u53ef\u4ee5\u73af\u5316\u4e3a\u786b\u80bd\u7684LazA\u7a81\u53d8\u4f53\u4ee5\u53ca\u7ebf\u578b\u5f62\u5f0f\u7684LazA\u7a81\u53d8\u4f53\u5e8f\u5217\uff0c\u5e76\u5c06\u5b83\u4eec\u4f5c\u4e3a\u6570\u636e\u96c6\u6765\u8bad\u7ec3\u53ef\u4ee5\u533a\u5206LazA\u7a81\u53d8\u4f53\u5e95\u7269\u9002\u5e94\u6027\u7684\u5377\u79ef\u795e\u7ecf\u7f51\u7edc\uff08Convolutional neural network\uff0cCNN\uff09\u3002\u6700\u7ec8\u6a21\u578b\u53ef\u4ee5\u8fbe\u52300.963\u7684\u51c6\u786e\u6027\u5e76\u4e14\u901a\u8fc7LC-MS\u9a8c\u8bc1\u4e86\u7ecf\u8fc7\u8bad\u7ec3\u7684\u6a21\u578b\u5bf9\u4e8eLazA\u7a81\u53d8\u4f53\u80bd\u7684\u5e95\u7269\u9002\u5e94\u6027\u7684\u4f30\u7b97\u662f\u53ef\u9760\u7684\u3002<\/p>\n<p>&nbsp;<\/p>\n<section><img decoding=\"async\" title=\"3\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20231124\/1700793918928289.png\" alt=\"3\" \/><\/section>\n<p>&nbsp;<\/p>\n<p>\u63a5\u4e0b\u6765\uff0c\u4f5c\u8005\u968f\u673a\u751f\u6210\u4e8610^4\u6761\u591a\u80bd\uff0c\u5e76\u4f7f\u7528\u8be5\u6a21\u578b\u8ba1\u7b97\u4e86\u5b83\u4eec\u7684\u5e73\u5747\u4fee\u9970\u6548\u7387\u3002\u9996\u5148\u9488\u5bf911\u805a\u4f53\u6587\u5e93\u8fdb\u884c\u8bbe\u8ba1\uff0c\u901a\u8fc7\u91c7\u7528GCG\uff08Ala\uff09\u66ff\u6362\u6bcf\u4e2aNNK\u7b80\u5e76\u5bc6\u7801\u5b50\u6765\u8fdb\u884cAla\u626b\u63cf\uff0c\u53d1\u73b0\u4f4d\u7f6e1\u548c11\u5bf9\u9884\u6d4bLazA\u7a81\u53d8\u4f53\u6210\u719f\u6548\u7387\u7684\u5f71\u54cd\u6700\u5927\uff0c\u901a\u8fc7\u8bc4\u4f30\u4f4d\u7f6e1\u548c11\u4e2d\u5df2\u8bc6\u522b\u5bc6\u7801\u5b50\u7684\u7ec4\u5408\uff0c\u7ed3\u679c\u8868\u660edsk-(nnk)9-nnu\u662f\u6700\u4f73\u7684\u8bbe\u8ba1\u3002\u5bf9\u4e8e\u8f83\u77ed\u7684\u6587\u5e93\uff0c\u7ed3\u679c\u8868\u660edbk-(nnk)n (n = 5\u22129) \u662f\u5f97\u5206\u6700\u9ad8\u7684\u8bbe\u8ba1\u3002<\/p>\n<p>&nbsp;<\/p>\n<section><img decoding=\"async\" title=\"7\" src=\"https:\/\/9794.seohost.cn\/storage\/9794\/article\/20231124\/1700793948774937.png\" alt=\"7\" \/><\/section>\n<p>&nbsp;<\/p>\n<p>\u6700\u540e\uff0c\u4f5c\u8005\u9488\u5bf9IRAK4\uff08\u5148\u5929\u708e\u75c7\u4fe1\u53f7\u901a\u8def\u4e2d\u7684\u5173\u952e\u6fc0\u9176\uff09\u548cTLR10\uff08\u4e0e\u5148\u5929\u514d\u75ab\u6709\u5173\u7684\u5b64\u513f\u53d7\u4f53\uff09\u8fd9\u4e24\u79cd\u86cb\u767d\u6d4b\u8bd5\u4e86\u5b83\u4eec\u4e0e\u786b\u80bd\u4eb2\u548c\u7684\u80fd\u529b\uff0c\u901a\u8fc7pulldown(+)\u548cpulldown (\u2212)\u5b9e\u9a8c\u7b5b\u9009\u5f97\u5230\u4e86\u9776\u5411IRAK4\u548cTLR10\u7684\u786b\u80bd\u3002\u5b9e\u9a8c\u8868\u660e\u53d1\u73b0\u9488\u5bf9IRAK4\u7684\u6700\u4f73\u5316\u5408\u7269\u7684KD\u503c\u9ad8\u8fbe1.3 nM\uff0c\u9488\u5bf9TLR10\u7684\u6700\u4f73\u5316\u5408\u7269KD\u503c\u9ad8\u8fbe300 nM\u3002IRAK4 \u9776\u5411\u5316\u5408\u7269\u8fd8\u5728\u4f53\u5916\u80fd\u591f\u4ee5\u4e2a\u4f4d\u6570\u7684\u03bcM\u6d53\u5ea6\u6291\u5236\u6fc0\u9176\uff0c\u53ef\u4ee5\u6709\u6548\u5185\u5316\u5230HEK293H\u7ec6\u80de\u4e2d\uff0c\u5e76\u6291\u5236\u7ec6\u80de\u4e2dNF-kB\u4ecb\u5bfc\u7684\u4fe1\u53f7\u4f20\u5bfc\u3002<\/p>\n<p>\u603b\u800c\u8a00\u4e4b\uff0c<strong>\u4f5c\u8005\u6240\u5f00\u53d1\u7684\u65b9\u6cd5\u7b80\u5316\u4e86\u5177\u6709\u91cd\u65b0\u8bbe\u8ba1\u7684\u751f\u7269\u6d3b\u6027\u548c\u6709\u5229\u836f\u7406\u5b66\u7279\u6027\u7684\u975e\u5929\u7136 RiPP \u7684\u53d1\u73b0\u3002<\/strong><\/p>\n<p>&nbsp;<\/p>\n<section>\u672c\u6587\u4f5c\u8005\uff1aCQS<\/section>\n<section>\u8d23\u4efb\u7f16\u8f91\uff1aWFZ<\/section>\n<section>\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/doi.org\/10.1021\/acscentsci.3c00957<\/section>\n<section>\u539f\u6587\u5f15\u7528\uff1aDOI\uff1a10.1021\/acscentsci.3c00957<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u63a8\u8350\u4e00\u7bc7\u53d1\u8868\u5728ACS Cent. Sci.\u4e0a\u7684\u8bba\u6587\uff0c\u9898\u76ee\u4e3a\u201cDeep Learning-Driven Libr [&hellip;]<\/p>\n","protected":false},"author":326,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-49537","post","type-post","status-publish","format-standard","hentry","category-tuijianyuedu"],"_links":{"self":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/49537","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/users\/326"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/comments?post=49537"}],"version-history":[{"count":1,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/49537\/revisions"}],"predecessor-version":[{"id":49538,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/posts\/49537\/revisions\/49538"}],"wp:attachment":[{"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/media?parent=49537"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/categories?post=49537"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chemhui.com\/wp-json\/wp\/v2\/tags?post=49537"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}